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Literature summary for 1.8.1.9 extracted from

  • Tamaki, S.; Maruta, T.; Sawa, Y.; Shigeoka, S.; Ishikawa, T.
    Biochemical and physiological analyses of NADPH-dependent thioredoxin reductase isozymes in Euglena gracilis (2015), Plant Sci., 236, 29-36 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Euglena gracilis

Protein Variants

Protein Variants Comment Organism
additional information expression as a truncated form without both the plastid-targetingpeptide and Trx domain Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
NADPH pH 7.2, temperature not specified in the publication Euglena gracilis
0.0224
-
NADPH pH 7.2, temperature not specified in the publication Euglena gracilis
0.0247
-
NADPH truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
0.0806
-
NADH truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
2.4
-
5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.2, temperature not specified in the publication Euglena gracilis
2.5
-
5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.2, temperature not specified in the publication Euglena gracilis
11.9
-
5,5'-dithio-bis(2-nitrobenzoic acid) truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Euglena gracilis 5829
-
mitochondrion
-
Euglena gracilis 5739
-
plastid
-
Euglena gracilis 9536
-

Organism

Organism UniProt Comment Textmining
Euglena gracilis W8VNN5 isoform Ntr1
-
Euglena gracilis W8VVL6 isoform NtrC
-
Euglena gracilis W8VZ54 isoform Ntr2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a putative very short mitochondria-targeting siglal peptide Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
0.92
-
pH 7.2, temperature not specified in the publication Euglena gracilis
7.32
-
pH 7.2, temperature not specified in the publication Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+
-
Euglena gracilis 5'-thionitrobenzoic acid + NAD+
-
?
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
Euglena gracilis 5'-thionitrobenzoic acid + NADP+
-
?

Subunits

Subunits Comment Organism
? x *34600, calculated Euglena gracilis
? x *51800, calculated Euglena gracilis
? x *53200, calculated Euglena gracilis

Synonyms

Synonyms Comment Organism
Ntr1
-
Euglena gracilis
NTR2
-
Euglena gracilis
Ntr3
-
Euglena gracilis
NTRC
-
Euglena gracilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
NADH truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
0.59
-
NADPH pH 7.2, temperature not specified in the publication Euglena gracilis
0.88
-
5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.2, temperature not specified in the publication Euglena gracilis
0.97
-
NADPH truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
1.23
-
5,5'-dithio-bis(2-nitrobenzoic acid) truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
6.4
-
NADPH pH 7.2, temperature not specified in the publication Euglena gracilis
10.7
-
5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.2, temperature not specified in the publication Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADH Euglena gracilis
NADH about 1% of the catalytic effciency with NADPH, truncated mutant Euglena gracilis
NADH about 2.5% of the activity with NADPH Euglena gracilis
NADPH
-
Euglena gracilis

General Information

General Information Comment Organism
physiological function growth rate of isoform Ntr1 mutants is significantly lower than that of control cells Euglena gracilis
physiological function growth rate of isoform Ntr2 mutants is significantly lower than that of control cells Euglena gracilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
5,5'-dithio-bis(2-nitrobenzoic acid) truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
0.38
-
5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.2, temperature not specified in the publication Euglena gracilis
2.48
-
NADH truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
4.33
-
5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.2, temperature not specified in the publication Euglena gracilis
26.5
-
NADPH pH 7.2, temperature not specified in the publication Euglena gracilis
398.5
-
NADPH truncated mutant, pH 7.2, temperature not specified in the publication Euglena gracilis
1220
-
NADPH pH 7.2, temperature not specified in the publication Euglena gracilis