Cloned (Comment) | Organism |
---|---|
- |
Euglena gracilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression as a truncated form without both the plastid-targetingpeptide and Trx domain | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
0.0224 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
0.0247 | - |
NADPH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
0.0806 | - |
NADH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
2.4 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
2.5 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
11.9 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Euglena gracilis | 5829 | - |
mitochondrion | - |
Euglena gracilis | 5739 | - |
plastid | - |
Euglena gracilis | 9536 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | W8VNN5 | isoform Ntr1 | - |
Euglena gracilis | W8VVL6 | isoform NtrC | - |
Euglena gracilis | W8VZ54 | isoform Ntr2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a putative very short mitochondria-targeting siglal peptide | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | - |
truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis |
0.92 | - |
pH 7.2, temperature not specified in the publication | Euglena gracilis |
7.32 | - |
pH 7.2, temperature not specified in the publication | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+ | - |
Euglena gracilis | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+ | - |
Euglena gracilis | 5'-thionitrobenzoic acid + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x *34600, calculated | Euglena gracilis |
? | x *51800, calculated | Euglena gracilis |
? | x *53200, calculated | Euglena gracilis |
Synonyms | Comment | Organism |
---|---|---|
Ntr1 | - |
Euglena gracilis |
NTR2 | - |
Euglena gracilis |
Ntr3 | - |
Euglena gracilis |
NTRC | - |
Euglena gracilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NADH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
0.59 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
0.88 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
0.97 | - |
NADPH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
1.23 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
6.4 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
10.7 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADH | Euglena gracilis | |
NADH | about 1% of the catalytic effciency with NADPH, truncated mutant | Euglena gracilis | |
NADH | about 2.5% of the activity with NADPH | Euglena gracilis | |
NADPH | - |
Euglena gracilis |
General Information | Comment | Organism |
---|---|---|
physiological function | growth rate of isoform Ntr1 mutants is significantly lower than that of control cells | Euglena gracilis |
physiological function | growth rate of isoform Ntr2 mutants is significantly lower than that of control cells | Euglena gracilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
0.38 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
2.48 | - |
NADH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
4.33 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
26.5 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
398.5 | - |
NADPH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
1220 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis |