KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
NADH | at pH 9.5 and 80°C | Thermotoga maritima | |
0.089 | - |
benzyl viologen | at pH 9.5 and 80°C | Thermotoga maritima | |
0.78 | - |
NADPH | at pH 6.5 and 80°C | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000, SDS-PAGE | Thermotoga maritima |
67000 | - |
gel filtration | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermotoga maritima | the enzyme cannot use thioredoxin from Spirulina as an electron acceptor | ? | - |
? | |
additional information | Thermotoga maritima DSM 3109 | the enzyme cannot use thioredoxin from Spirulina as an electron acceptor | ? | - |
? | |
thioredoxin disulfide + NADPH + H+ | Thermotoga maritima | - |
thioredoxin + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | Thermotoga maritima DSM 3109 | - |
thioredoxin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Thermotoga maritima DSM 3109 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+ | - |
Thermotoga maritima | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+ | - |
Thermotoga maritima DSM 3109 | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+ | - |
Thermotoga maritima | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+ | - |
Thermotoga maritima DSM 3109 | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
benzyl viologen + NADH + H+ | - |
Thermotoga maritima | ? | - |
? | |
benzyl viologen + NADH + H+ | - |
Thermotoga maritima DSM 3109 | ? | - |
? | |
benzyl viologen + NADPH + H+ | - |
Thermotoga maritima | ? | - |
? | |
additional information | the enzyme cannot use thioredoxin from Spirulina as an electron acceptor | Thermotoga maritima | ? | - |
? | |
additional information | the enzyme cannot use thioredoxin from Spirulina as an electron acceptor | Thermotoga maritima DSM 3109 | ? | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Thermotoga maritima | thioredoxin + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Thermotoga maritima DSM 3109 | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 31000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
L-TR | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 95 | enzyme activity increases along with the rise of temperature up to 95°C, and more than 60% of the activity remains after incubation for 28 h at 80°C and 50% remains after 9 h at 95°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
with NADPH as cosubstrate | Thermotoga maritima |
9.5 | - |
with NADH as cosubstrate | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1.88 mol of FAD per mol native enzyme | Thermotoga maritima | |
NADH | the enzyme shows a preference for NADH over NADPH | Thermotoga maritima | |
NADPH | - |
Thermotoga maritima |