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Literature summary for 1.8.1.9 extracted from

  • Yang, X.; Ma, K.
    Characterization of a thioredoxin-thioredoxin reductase system from the hyperthermophilic bacterium Thermotoga maritima (2010), J. Bacteriol., 192, 1370-1376.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
NADH at pH 9.5 and 80°C Thermotoga maritima
0.089
-
benzyl viologen at pH 9.5 and 80°C Thermotoga maritima
0.78
-
NADPH at pH 6.5 and 80°C Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
2 * 31000, SDS-PAGE Thermotoga maritima
67000
-
gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermotoga maritima the enzyme cannot use thioredoxin from Spirulina as an electron acceptor ?
-
?
additional information Thermotoga maritima DSM 3109 the enzyme cannot use thioredoxin from Spirulina as an electron acceptor ?
-
?
thioredoxin disulfide + NADPH + H+ Thermotoga maritima
-
thioredoxin + NADP+
-
?
thioredoxin disulfide + NADPH + H+ Thermotoga maritima DSM 3109
-
thioredoxin + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima DSM 3109
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+
-
Thermotoga maritima 5'-thionitrobenzoic acid + NAD+
-
?
5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+
-
Thermotoga maritima DSM 3109 5'-thionitrobenzoic acid + NAD+
-
?
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
Thermotoga maritima 5'-thionitrobenzoic acid + NADP+
-
?
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
Thermotoga maritima DSM 3109 5'-thionitrobenzoic acid + NADP+
-
?
benzyl viologen + NADH + H+
-
Thermotoga maritima ?
-
?
benzyl viologen + NADH + H+
-
Thermotoga maritima DSM 3109 ?
-
?
benzyl viologen + NADPH + H+
-
Thermotoga maritima ?
-
?
additional information the enzyme cannot use thioredoxin from Spirulina as an electron acceptor Thermotoga maritima ?
-
?
additional information the enzyme cannot use thioredoxin from Spirulina as an electron acceptor Thermotoga maritima DSM 3109 ?
-
?
thioredoxin disulfide + NADPH + H+
-
Thermotoga maritima thioredoxin + NADP+
-
?
thioredoxin disulfide + NADPH + H+
-
Thermotoga maritima DSM 3109 thioredoxin + NADP+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 31000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
L-TR
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80 95 enzyme activity increases along with the rise of temperature up to 95°C, and more than 60% of the activity remains after incubation for 28 h at 80°C and 50% remains after 9 h at 95°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
with NADPH as cosubstrate Thermotoga maritima
9.5
-
with NADH as cosubstrate Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
FAD 1.88 mol of FAD per mol native enzyme Thermotoga maritima
NADH the enzyme shows a preference for NADH over NADPH Thermotoga maritima
NADPH
-
Thermotoga maritima