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Literature summary for 1.8.1.9 extracted from

  • Dai, C.; Wang, M.H.
    Isolation and characterization of thioredoxin and NADPH-dependent thioredoxin reductase from tomato (Solanum lycopersicum) (2011), BMB Rep., 44, 692-697.
    View publication on PubMed
Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00031
-
 thioredoxin in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
0.00235
-
 NADPH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
0.0186
-
 5,5'-dithiobis(2-nitrobenzoic acid) in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
0.1
-
 NADH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
 x * 36500, calculated from amino acid sequence Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thioredoxin + NADP+ Solanum lycopersicum
-
 thioredoxin disulfide + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity column chromatography Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Solanum lycopersicum 5'-thionitrobenzoic acid + NAD+
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Solanum lycopersicum 5'-thionitrobenzoic acid + NADP+
-
 ?
thioredoxin + NADP+
-
 Solanum lycopersicum thioredoxin disulfide + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 36500, calculated from amino acid sequence Solanum lycopersicum

Synonyms

Synonyms Comment Organism
NADPH-dependent thioredoxin reductase
-
 Solanum lycopersicum
NTR
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 NADH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
0.35
-
 NADPH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
0.38
-
 thioredoxin in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.77
-
 5,5'-dithiobis(2-nitrobenzoic acid) in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Solanum lycopersicum
NADPH the catalytic efficiency with NADPH is 2500fold higher than with NADH Solanum lycopersicum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum lycopersicum calculated from amino acid sequence
-
 5.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
 NADH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
95
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
150
-
 NADPH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1230
-
 thioredoxin in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum