Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Entamoeba histolytica | |
NAD+ | NAD+ acts as poor competitive inhibitor respect to both NADPH and NADH | Entamoeba histolytica | |
NADP+ | better competitive inhibitor than NAD+ | Entamoeba histolytica | |
Zn2+ | - |
Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
NADPH | with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.0023 | - |
thioredoxin 41 | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.0028 | - |
thioredoxin 8 | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.0029 | - |
thioredoxin 8 | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.0036 | - |
thioredoxin 41 | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.0047 | - |
thioredoxin | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.0062 | - |
thioredoxin | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.023 | - |
NADH | with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
1.1 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
1.4 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
2 * 64000, SDS-PAGE | Entamoeba histolytica |
78000 | - |
gel filtration | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Entamoeba histolytica | in addition, the enzyme exhibits NAD(P)H dependent oxidase activity, which generates hydrogen peroxide from molecular oxygen | ? | - |
? | |
thioredoxin + NADP+ | Entamoeba histolytica | - |
thioredoxin disulfide + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+ | - |
Entamoeba histolytica | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Entamoeba histolytica | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
additional information | in addition, the enzyme exhibits NAD(P)H dependent oxidase activity, which generates hydrogen peroxide from molecular oxygen | Entamoeba histolytica | ? | - |
? | |
thioredoxin + NADP+ | - |
Entamoeba histolytica | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin 41 + NADP+ | - |
Entamoeba histolytica | thioredoxin 41 disulfide + NADPH + H+ | - |
? | |
thioredoxin 8 + NADP+ | - |
Entamoeba histolytica | thioredoxin 8 disulfide + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 64000, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
TrxR | - |
Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NADH | with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.23 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.25 | - |
NADPH | with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.25 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.5 | - |
thioredoxin | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
1.25 | - |
thioredoxin | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
2 | - |
thioredoxin 41 | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
2.2 | - |
thioredoxin 41 | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
2.6 | - |
thioredoxin 8 | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
2.7 | - |
thioredoxin 8 | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol FAD is bound per mol subunit | Entamoeba histolytica | |
NADH | - |
Entamoeba histolytica | |
NADPH | higher affinity and catalytic efficiency with NADPH compared to NADH | Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NADP+ | in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.026 | - |
NADP+ | in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.313 | - |
NAD+ | in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
1.478 | - |
NAD+ | in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0025 | - |
in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | Cu2+ | |
0.0034 | - |
in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | Zn2+ |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
0.23 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
75 | - |
thioredoxin | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
84 | - |
NADH | with 5,5'-dithiobis(2-nitrobenzoicacid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
140 | - |
NADPH | with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
260 | - |
thioredoxin | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
600 | - |
thioredoxin 41 | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
880 | - |
thioredoxin 41 | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
890 | - |
thioredoxin 8 | with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica | |
950 | - |
thioredoxin 8 | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C | Entamoeba histolytica |