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Literature summary for 1.8.1.9 extracted from
- Insights into the specificity of thioredoxin reductase-thioredoxin interactions. A structural and functional investigation of the yeast thioredoxin system (2010), Biochemistry, 49, 3317-3326.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D146A | inactive | Saccharomyces cerevisiae |
| K137A | the mutation does not alter enzyme activity | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
thioredoxin 2 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.0009 | - |
thioredoxin 2 | mutant enzyme K137A, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.0011 | - |
thioredoxin 3 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.0013 | - |
thioredoxin 1 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, X-ray crystallography | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | the yeast enzyme fails to reduce the human and Escherichia coli thioredoxin | ? | - |
? | |
| thioredoxin 1 + NADP+ + | Saccharomyces cerevisiae | - |
thioredoxin 1 disulfide + NADPH + H+ | - |
? | |
| thioredoxin 2 + NADP+ | Saccharomyces cerevisiae | - |
thioredoxin 2 disulfide + NADPH + H+ | - |
? | |
| thioredoxin 3 + NADP+ | Saccharomyces cerevisiae | - |
thioredoxin 3 disulfide + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the yeast enzyme fails to reduce the human and Escherichia coli thioredoxin | Saccharomyces cerevisiae | ? | - |
? | |
| thioredoxin 1 + NADP+ + | - |
Saccharomyces cerevisiae | thioredoxin 1 disulfide + NADPH + H+ | - |
? | |
| thioredoxin 2 + NADP+ | - |
Saccharomyces cerevisiae | thioredoxin 2 disulfide + NADPH + H+ | - |
? | |
| thioredoxin 3 + NADP+ | - |
Saccharomyces cerevisiae | thioredoxin 3 disulfide + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 35000, X-ray crystallography | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrxR1 | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34 | - |
thioredoxin 3 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 42.9 | - |
thioredoxin 2 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 43.7 | - |
thioredoxin 1 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 47.1 | - |
thioredoxin 2 | mutant enzyme K137A, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Saccharomyces cerevisiae | |
| NADP+ | - |
Saccharomyces cerevisiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31000 | - |
thioredoxin 3 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 34000 | - |
thioredoxin 1 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 52000 | - |
thioredoxin 2 | mutant enzyme K137A, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 73000 | - |
thioredoxin 2 | wild type enzyme, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
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