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Literature summary for 1.8.1.9 extracted from
- Identification and characterization of a selenoprotein, thioredoxin reductase, in a unicellular marine haptophyte alga, Emiliania huxleyi (2008), J. Biol. Chem., 283, 35329-35336.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52320 | - |
isoform SEP1, calculated from amino acid sequence | Emiliania huxleyi |
| 59000 | - |
isoform SEP1, SDS-PAGE | Emiliania huxleyi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Emiliania huxleyi | B9A1H3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Toyopearl column chromatography and Diol-150 gel filtration | Emiliania huxleyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.1 | - |
using thioredoxin disulfide and NADPH as substrates, at 25°C | Emiliania huxleyi |
| 36.4 | - |
using thioredoxin disulfide, insulin, and NADPH as substrates, at 25°C | Emiliania huxleyi |
| 150.6 | - |
using 5,5'-dithiobis(2-nitrobenzoic acid) and NADPH as substrates, at 25°C | Emiliania huxleyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Emiliania huxleyi | 2-nitro-5-thiobenzoate + NADP+ | - |
? |  |
| thioredoxin + NADP+ | - |
Emiliania huxleyi | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin disulfide + NADPH + H+ | - |
Emiliania huxleyi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52320, isoform SEP1, calculated from amino acid sequence | Emiliania huxleyi |
| ? | x * 59000, isoform SEP1, SDS-PAGE | Emiliania huxleyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SEP1 | isoform | Emiliania huxleyi |
| thioredoxin reductase | - |
Emiliania huxleyi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Emiliania huxleyi | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Emiliania huxleyi | thioredoxin reductase activity gradually increases 4fold within ca. 70 h when cells are transferred to the medium containing 10 nM selenite, selenium is essential for the induction of thioredoxin reductase activity at the translational level but not at the transcriptional level | up |
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