Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acidithiobacillus ferrooxidans |
Protein Variants | Comment | Organism |
---|---|---|
C142A | mutant shows reduced reductase activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
C142S | mutant shows reduced reductase activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
C145A | mutant shows reduced reductase activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
C145S | mutant shows reduced reductase activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
SDS-PAGE | Acidithiobacillus ferrooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
strain ATCC 23270 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Acidithiobacillus ferrooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.1 | - |
mutant enzyme C145S, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
5.5 | - |
mutant enzyme C142S, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
5.6 | - |
mutant enzyme C145A, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
6.4 | - |
mutant enzyme C142A, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
29.7 | - |
wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 20°C | Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin disulfide + NADPH + H+ | - |
Acidithiobacillus ferrooxidans | thioredoxin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thioredoxin reductase | - |
Acidithiobacillus ferrooxidans |
TrxR | - |
Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Acidithiobacillus ferrooxidans | |
NADPH | - |
Acidithiobacillus ferrooxidans |