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Literature summary for 1.8.1.9 extracted from
- Thioredoxin-linked metabolism in Entamoeba histolytica (2007), Free Radic. Biol. Med., 42, 1496-1505.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Entamoeba histolytica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
Entamoeba histolytica thioredoxin disulfide 41 | pH 7.0, 25°C | Entamoeba histolytica | |
| 0.0045 | - |
NADPH | pH 7.0, 25°C, cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid) | Entamoeba histolytica |  |
| 0.0046 | - |
Escherichia coli thioredoxin disulfide | pH 7.0, 25°C | Entamoeba histolytica | |
| 1.7 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | pH 7.0, 25°C | Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33700 | - |
x * 33700, calculated from sequence | Entamoeba histolytica |
| 36000 | - |
x * 36000, SDS-PAGE | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Entamoeba histolytica | EhTRXR and EhTRX41 could be assayed as a functional redox pair that, together with peroxiredoxin, mediates the NADPH-dependent reduction of hydrogen peroxide and tert-butyl hydroperoxide. It is proposed that this detoxifying system could be operative in vivo | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Entamoeba histolytica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| trophozoite | - |
Entamoeba histolytica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Entamoeba histolytica | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| Entamoeba histolytica thioredoxin disulfide 41 + NADPH + H+ | - |
Entamoeba histolytica | Entamoeba histolytica thioredoxin 41 + NADP+ | - |
? | |
| Escherichia coli thioredoxin disulfide + NADPH + H+ | - |
Entamoeba histolytica | Escherichia coli thioredoxin + NADP+ | - |
? | |
| additional information | EhTRXR and EhTRX41 could be assayed as a functional redox pair that, together with peroxiredoxin, mediates the NADPH-dependent reduction of hydrogen peroxide and tert-butyl hydroperoxide. It is proposed that this detoxifying system could be operative in vivo | Entamoeba histolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Entamoeba histolytica |
| ? | x * 33700, calculated from sequence | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EhTRXR | - |
Entamoeba histolytica |
| thioredoxin reductase | - |
Entamoeba histolytica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Entamoeba histolytica | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Entamoeba histolytica | calculated from sequence | - |
5.94 |
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