Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | C-terminal redox active disulfide | Drosophila melanogaster |
Cloned (Comment) | Organism |
---|---|
expression of cytosolic and mitochondrial isoform as His-tagged enzymes in Escherichia coli | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | His-tagged transcript specific mutants, varied N-terminus, 1 null-mutant | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NADPH | cytosolic and mitochondrial TrxR1 isoform | Drosophila melanogaster | |
0.007 | - |
thioredoxin | cytosolic TrxR1 isoform | Drosophila melanogaster | |
0.007 | - |
thioredoxin-1 disulfide | TrxR(cyto) | Drosophila melanogaster | |
0.019 | - |
thioredoxin | mitochondrial TrxR1 isoform | Drosophila melanogaster | |
0.019 | - |
thioredoxin-1 disulfide | TRxR(mito) | Drosophila melanogaster | |
0.38 | - |
DTNB | cytosolic TrxR1 isoform | Drosophila melanogaster | |
0.41 | - |
DTNB | mitochondrial TrxR1 isoform | Drosophila melanogaster | |
1 | - |
NADPH | TrxR-1(cyto) | Drosophila melanogaster | |
1 | - |
NADPH | TrxR-1(mito) | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | TrxR-1(cyto) | Drosophila melanogaster | 5737 | - |
cytosol | isoform TrxR1 | Drosophila melanogaster | 5829 | - |
mitochondrion | isoform TrxR3 and TrxR1 | Drosophila melanogaster | 5739 | - |
mitochondrion | TrxR-1(mito) | Drosophila melanogaster | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | TrxR-1(cyto) is not a selenoprotein | Drosophila melanogaster | |
additional information | TrxR-1(mito) is not a selenoprotein | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53200 | - |
cytosolic TrxR1 isoform, deduced from amino acid sequence | Drosophila melanogaster |
63700 | - |
mitochondrial TrxR1 isoform, deduced from amino acid sequence | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + NADP+ | Drosophila melanogaster | defense against oxidative stress | thioredoxin disulfide + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Drosophila melanogaster | - |
3 isoforms TrxR1, TrxR2, TrxR3, encoded by 3 genes | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type cytosolic and mitochondrial isoforms and mutants from Escherichia coli | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Drosophila melanogaster | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | defense against oxidative stress | Drosophila melanogaster | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin-1 disulfide + NADPH | - |
Drosophila melanogaster | NADP+ + thioredoxin-1 | - |
? | |
thioredoxin-1 disulfide + NADPH + H+ | - |
Drosophila melanogaster | thioredoxin-1 + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 53200, TrxR-1(cyto), calculation from nucleotide sequence | Drosophila melanogaster |
? | x * 63700, TrxR-1(mito), calculation from nucleotide sequence | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
TrxR | - |
Drosophila melanogaster |
TrxR-1(cyto) | - |
Drosophila melanogaster |
TrxR-1(mito) | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
cytosolic and mitochondrial TrxR1 isoform | Drosophila melanogaster |
7.1 | - |
TrxR-1(cyto) | Drosophila melanogaster |
7.1 | - |
TrxR-1(mito) | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Drosophila melanogaster | |
NADPH | - |
Drosophila melanogaster |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Drosophila melanogaster | TrxR-1(cyto) | - |
5.93 |
Drosophila melanogaster | TrxR-1(mito) | - |
8.15 |