Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | part of the active site | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenocysteine | contains selenocysteine as part of the redox active selenosulfide in the active center | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
HeLa cells | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | high and low affinity form with respect to heparin | Homo sapiens | - |
lung | adenocarcinoma cells, high and low affinity form with respect to heparin | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | in presence of NADPH, coupled assay | Homo sapiens | thioredoxin disulfide + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, SDS-PAGE | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |