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Literature summary for 1.8.1.9 extracted from
- A stable mixed disulfide between thioredoxin reductase and its substrate, thioredoxin: preparation and characterization (1996), Biochemistry, 35, 4812-4819.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subunit complex of enzyme via C135 with thioredoxin C32, mutant complex S135-S32, expression from plasmid | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C135S/C32S | via the active disulfide centers a subunit complex of tightly bound enzyme, C135 and C138, and thioredoxin, C32 and C35, is formed, exchange of one cysteine for one serine in each protein by site-directed mutagenesis, conformation analysis | Escherichia coli |
| C535S | site-directed mutagenesis, changed conformation | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme-thioredoxin subunit complex C135-C32 and mutant S135-S32 | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | electron transfer in the enzyme complex of apoenzyme, FAD and thioredoxin with NADPH | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors | Escherichia coli | ? | - |
? |  |
| thioredoxin + 3-acetylpyridine adenine dinucleotide | wild-type enzyme, mutant enzyme C135S and thioredoxin in subunit complex C135-C32S with the enzyme | Escherichia coli | thioredoxin disulfide + reduced 3-acetylpyridine adenine dinucleotide | - |
? | |
| thioredoxin + NADP+ | - |
Escherichia coli | thioredoxin disulfide + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrR | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | transhydrogenase activity of mutants and wild-type enzyme with thioredoxin and FAD | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 3-acetylpyridine adenine dinucleotide | - |
Escherichia coli | |
| FAD | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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