Literature summary for 1.8.1.9 extracted from

Gleason, F.K.; Lim, C.J.; Gerami-Nejad, M.; Fuchs, J.A.
Characterization of Escherichia coli thioredoxins with altered active site residues (1990), Biochemistry, 29, 3701-3709.

Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
trxB gene, cloning of active site mutants, expression from plasmids	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km-values are pH-dependent	Escherichia coli
0.002	-	thioredoxin	substrate wild-type, thioredoxin P34S, pH 8.0	Escherichia coli
0.0067	-	thioredoxin K36R	substrate mutant, pH 8.0	Escherichia coli
0.125	-	thioredoxin-R	substrate mutant, thioredoxin-CAC, pH 8.0	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	overproducing strain, trxB gene in a pUC vector	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type from overexpressing cell line, mutants	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+	oxidation-reduction cycle of thioredoxin	Escherichia coli	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	3 different assay methods	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thioredoxin + NADP+	-	Escherichia coli	thioredoxin disulfide + NADPH	-	ir
thioredoxin K36E + NADP+	-	Escherichia coli	thioredoxin K36E disulfide + NADPH + H+	-	r
thioredoxin K36R + NADP+	-	Escherichia coli	thioredoxin K36R disulfide + NADPH + H+	-	r
thioredoxin P34S + NADP+	-	Escherichia coli	thioredoxin P34S disulfide + NADPH + H+	-	r
thioredoxin-CAC + NADP+	-	Escherichia coli	thioredoxin-CAC disulfide + NADPH + H+	-	r
thioredoxin-R + NADP+	-	Escherichia coli	thioredoxin-R disulfide + NADPH + H+	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10.3	-	thioredoxin-R	mutant, pH 8.0	Escherichia coli
10.3	-	thioredoxin-CAC	mutant, pH 8.0	Escherichia coli
22.7	-	thioredoxin K36E	mutant, pH 8.0	Escherichia coli
22.7	-	thioredoxin P34S	mutant, pH 8.0	Escherichia coli
22.8	-	thioredoxin	wild-type, pH 8.0	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Escherichia coli
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)