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Literature summary for 1.8.1.9 extracted from
- Flavin-containing dehydrogenases (1976), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 13, 89-173.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | 2fold activation | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | product inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
NADPH | at 4°C | Escherichia coli | |
| 0.0012 | - |
NADPH | at 25°C | Escherichia coli | |
| 0.0017 | - |
thioredoxin | at 4°C | Escherichia coli | |
| 0.0028 | - |
thioredoxin | at 25°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
sedimentation equilibrium analysis | Escherichia coli |
| 73000 | 75000 | amino acid analysis based on 2 mol FAD per molecule of enzyme | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thioredoxin + NADP+ | Escherichia coli | metabolic function of thioredoxin reductase-thioredoxin system: supplies reducing equivalents for a wide variety of acceptors, e.g. : ribonucleotide reductase, nonspecific protein disulfide reductase, methionine sulfoxide reductase, D-proline reductase | thioredoxin disulfide + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | catalytic mechanism | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | reduction of thioredoxin by NADPH is virtually complete, equilibrium constant is 48 at pH 7 | Escherichia coli | ? | - |
? | |
| additional information | reduction of thioredoxin by NADPH is virtually complete, equilibrium constant is 48 at pH 7 | Escherichia coli B / ATCC 11303 | ? | - |
? | |
| additional information | reduction of thioredoxin by NADPH is virtually complete, equilibrium constant is 48 at pH 7 | Escherichia coli Crookes | ? | - |
? | |
| additional information | reduction of thioredoxin by NADPH is virtually complete, equilibrium constant is 48 at pH 7 | Escherichia coli M191-6 | ? | - |
? | |
| thioredoxin + NADP+ | wide variety of electron acceptors | Escherichia coli | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | metabolic function of thioredoxin reductase-thioredoxin system: supplies reducing equivalents for a wide variety of acceptors, e.g. : ribonucleotide reductase, nonspecific protein disulfide reductase, methionine sulfoxide reductase, D-proline reductase | Escherichia coli | thioredoxin disulfide + NADPH | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
about | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 2 FAD per dimer | Escherichia coli | |
| NADPH | specific for | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
NADP+ | at 25°C | Escherichia coli | |
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