BRENDA - Enzyme Database show
show all sequences of 1.8.1.8

Purification and characterization of a Bacillus megaterium disulfide reductase specific for disulfides containing pantethine 4',4'-diphosphate

Swerdlow, R.D.; Setlow, P.; J. Bacteriol. 153, 475-484 (1983)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
-
Bacillus megaterium
N-ethylmaleimide
-
Bacillus megaterium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.65
-
pantethine 4',4''-diphosphate
-
Bacillus megaterium
7.4
-
CoA-SS-CoA
-
Bacillus megaterium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
2 * 55000, SDS-PAGE
Bacillus megaterium
122000
-
gel filtration
Bacillus megaterium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus megaterium
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bacillus megaterium
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
the enzyme is at a low level in log-phase cells but increases up to 10fold early in the stationary phase and has a similar specific activity in both the mother cell and the forespore compartment. The enzyme activity falls only slowly during spore germination and outgrowth
Bacillus megaterium
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is active only on disulfides containing pantethine 4',4"-diphosphate moieties, including pantethine 4',4"-diphosphate, oxidized coenzyme A, and coenzyme A in disulfide linkage to acyl carrier protein
680424
Bacillus megaterium
?
-
-
-
-
NADH + CoA-SS-CoA + H+
-
680424
Bacillus megaterium
NAD+ + CoA
-
-
-
?
NADH + pantethine 4',4''-diphosphate
-
680424
Bacillus megaterium
NAD+ + 4'-phosphopantetheine
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 55000, SDS-PAGE
Bacillus megaterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
substrate: pantethine 4',4''-diphosphate
Bacillus megaterium
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
seven times as active in phosphate buffer as in Tris at the same pH
Bacillus megaterium
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains one molecule of flavin adenine dinucleotide per subunit
Bacillus megaterium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains one molecule of flavin adenine dinucleotide per subunit
Bacillus megaterium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
-
Bacillus megaterium
N-ethylmaleimide
-
Bacillus megaterium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.65
-
pantethine 4',4''-diphosphate
-
Bacillus megaterium
7.4
-
CoA-SS-CoA
-
Bacillus megaterium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
2 * 55000, SDS-PAGE
Bacillus megaterium
122000
-
gel filtration
Bacillus megaterium
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus megaterium
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
the enzyme is at a low level in log-phase cells but increases up to 10fold early in the stationary phase and has a similar specific activity in both the mother cell and the forespore compartment. The enzyme activity falls only slowly during spore germination and outgrowth
Bacillus megaterium
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is active only on disulfides containing pantethine 4',4"-diphosphate moieties, including pantethine 4',4"-diphosphate, oxidized coenzyme A, and coenzyme A in disulfide linkage to acyl carrier protein
680424
Bacillus megaterium
?
-
-
-
-
NADH + CoA-SS-CoA + H+
-
680424
Bacillus megaterium
NAD+ + CoA
-
-
-
?
NADH + pantethine 4',4''-diphosphate
-
680424
Bacillus megaterium
NAD+ + 4'-phosphopantetheine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 55000, SDS-PAGE
Bacillus megaterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
substrate: pantethine 4',4''-diphosphate
Bacillus megaterium
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
seven times as active in phosphate buffer as in Tris at the same pH
Bacillus megaterium
Other publictions for EC 1.8.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
1
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1
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1
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1
1
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3
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1
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6
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8
1
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1
2
1
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3
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1
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8
1
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743076
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-
-
-
-
-
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-
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1
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1
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1
-
-
-
-
-
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742467
Pedone
Functional and structural cha ...
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
Extremophiles
18
723-731
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
9
-
-
-
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4
1
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1
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1
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4
1
-
-
-
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-
-
-
1
-
-
-
-
-
-
725973
Hagiwara
Structural basis of an ERAD pa ...
Mus musculus
Mol. Cell
41
432-444
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
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-
1
-
-
-
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-
1
1
1
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1
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1
1
-
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-
745744
Scir
High hydrostatic pressure-ind ...
Pyrococcus furiosus
Mol. Biosyst.
6
2015-2022
2010
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
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-
-
-
-
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-
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1
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-
687756
Jeong
ERp16, an endoplasmic reticulu ...
Homo sapiens
J. Biol. Chem.
283
25557-25566
2008
-
-
1
-
-
-
-
-
1
-
2
-
-
2
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
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-
1
1
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1
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2
-
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-
1
-
1
-
-
1
1
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-
-
-
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-
-
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-
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-
670797
Garg
Characterization of Mycobacter ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis Rv3219
Protein Expr. Purif.
52
422-432
2007
-
-
1
-
-
-
-
-
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2
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4
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1
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1
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2
1
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1
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1
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1
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2
1
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-
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687393
Lessner
The archaeon Methanosarcina ac ...
Methanosarcina acetivorans
J. Bacteriol.
189
7475-7484
2007
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-
1
-
-
-
-
-
-
1
-
1
-
6
-
-
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-
-
-
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-
2
-
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-
1
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1
1
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1
-
1
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-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
722206
Kuroita
Functional similarities of a t ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
Extremophiles
11
85-94
2007
-
-
1
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
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-
1
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1
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1
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1
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1
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1
-
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671296
Tomazzolli
A fluorescence-based assay for ...
Homo sapiens
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350
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2006
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-
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-
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1
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673557
Ladenstein
Protein disulfides and protein ...
Pyrococcus furiosus, Pyrococcus horikoshii, Saccharolobus solfataricus, Thermococcus kodakarensis, Thermotoga maritima
FEBS J.
273
4170-4185
2006
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
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-
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7
2
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8
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8
-
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1
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7
2
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-
-
-
-
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-
-
-
-
-
677156
Maeda
Structural basis for target pr ...
Hordeum vulgare
Structure
14
1701-1710
2006
-
-
1
1
-
-
-
-
-
-
-
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-
2
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1
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1
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1
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1
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657677
Poole
Bacterial defenses against oxi ...
Salmonella enterica subsp. enterica serovar Typhimurium
Arch. Biochem. Biophys.
433
240-254
2005
-
-
-
1
1
-
1
-
-
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-
2
-
2
-
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5
1
1
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2
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2
1
1
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1
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2
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5
1
1
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745638
Pedone
Temperature-, SDS-, and pH-in ...
Pyrococcus furiosus
J. Proteome Res.
4
1972-1980
2005
-
-
-
-
-
1
-
-
-
-
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-
1
1
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1
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1
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3
-
1
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3
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1
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3
-
1
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3
-
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-
659424
Argyrou
Characterization of a new memb ...
Mycobacterium tuberculosis
J. Biol. Chem.
279
52694-52702
2004
-
-
1
1
-
-
2
11
-
-
-
1
-
4
-
-
1
1
-
-
-
-
6
1
1
-
-
12
1
-
-
2
2
-
-
-
-
1
2
1
-
-
-
2
2
11
-
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-
1
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1
-
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-
6
1
1
-
-
12
1
-
-
-
-
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-
723100
Bartolucci
Protein disulfide oxidoreducta ...
Pyrococcus furiosus
Methods Enzymol.
334
62-73
2001
-
-
1
-
-
-
-
-
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-
2
-
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1
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1
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1
1
1
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1
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1
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1
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2
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1
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1
1
1
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1
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1
-
-
-
-
-
-
723101
Ren
Protein disulfide oxidoreducta ...
Pyrococcus furiosus
Methods Enzymol.
334
74-88
2001
-
-
-
1
-
-
-
-
-
1
-
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1
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1
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1
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680424
Swerdlow
Purification and characterizat ...
Bacillus megaterium
J. Bacteriol.
153
475-484
1983
-
-
-
-
-
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2
2
-
-
2
-
-
6
-
-
1
-
-
2
-
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3
1
-
-
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-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
2
-
-
-
-
1
-
2
-
-
3
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
680588
Swerdlow
Identification of an NADH-link ...
Bacillus megaterium
J. Biol. Chem.
254
6835-6837
1979
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
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4
-
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1
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1
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4
-
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437710
Hatch
A protein disulfide reductase ...
Avena sativa, Hordeum vulgare, Pisum sativum, Triticum aestivum, Vicia faba, Zea mays
Biochem. J.
76
556-562
1960
-
-
-
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8
1
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6
-
-
1
-
-
14
1
6
6
-
-
-
2
-
1
1
1
2
-
-
-
-
-
-
2
-
-
-
-
8
-
1
-
-
-
-
-
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-
1
-
14
1
6
6
-
-
-
2
-
1
1
1
-
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