BRENDA - Enzyme Database show
show all sequences of 1.8.1.8

A protein disulfide reductase from pea seeds

Hatch, M.D.; Turner, J.F.; Biochem. J. 76, 556-562 (1960)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
AgNO3
0.009 mM, 66% inhibition
Pisum sativum
arsenite
0.15 mM, complete inhibition
Pisum sativum
CdSO4
0.005 mM, 91% inhibition
Pisum sativum
CuSO4
0.015 mM, complete inhibition
Pisum sativum
HgCl2
0.002 mM, 92% inhibition
Pisum sativum
iodoacetate
0.054 mM, 57% inhibition
Pisum sativum
p-chloromercuribenzoate
0.054 mM, 88% inhibition
Pisum sativum
ZnSO4
0.003 mM, 82% inhibition
Pisum sativum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
NADPH
-
Pisum sativum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Avena sativa
-
-
-
Hordeum vulgare
-
-
-
Pisum sativum
-
-
-
Triticum aestivum
-
-
-
Vicia faba
-
-
-
Zea mays
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pisum sativum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Hordeum vulgare
-
root
-
Pisum sativum
-
root
-
Zea mays
-
seed
-
Avena sativa
-
seed
-
Hordeum vulgare
-
seed
-
Pisum sativum
-
seed
-
Triticum aestivum
-
seed
-
Vicia faba
-
seed
-
Zea mays
-
shoot
-
Hordeum vulgare
-
shoot
-
Pisum sativum
-
shoot
-
Triticum aestivum
-
shoot
-
Vicia faba
-
shoot
-
Zea mays
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pisum sativum
Storage Stability
Storage Stability
Organism
-15°C, 8 weeks, stable
Vicia faba
-15°C, 8 weeks, stable
Triticum aestivum
-15°C, 8 weeks, stable
Hordeum vulgare
-15°C, 8 weeks, stable
Pisum sativum
-15°C, 8 weeks, stable
Zea mays
-15°C, 8 weeks, stable
Avena sativa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD(P)H + protein disulfide
-
437710
Vicia faba
NAD(P)+ + protein dithiol
-
437710
Vicia faba
?
NAD(P)H + protein disulfide
-
437710
Triticum aestivum
NAD(P)+ + protein dithiol
-
437710
Triticum aestivum
?
NAD(P)H + protein disulfide
-
437710
Hordeum vulgare
NAD(P)+ + protein dithiol
-
437710
Hordeum vulgare
?
NAD(P)H + protein disulfide
-
437710
Zea mays
NAD(P)+ + protein dithiol
-
437710
Zea mays
?
NAD(P)H + protein disulfide
-
437710
Avena sativa
NAD(P)+ + protein dithiol
-
437710
Avena sativa
?
NAD(P)H + protein disulfide
reaction rate with NADPH is approximately twice that with NADH
437710
Pisum sativum
NAD(P)+ + protein dithiol
-
437710
Pisum sativum
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
10 min, stable
Pisum sativum
70
-
10 min, complete inactivation
Pisum sativum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
7.4
-
Pisum sativum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
7.8
pH 6.2: about 50% of activity maximum, pH 7.8: about 20% of activity maximum
Pisum sativum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
-
5°C or 22°C, irreversible inactivation
Pisum sativum
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
reaction rate with NADPH is approximately twice of that with NADH
Pisum sativum
NADPH
reaction rate with NADPH is approximately twice of that with NADH
Pisum sativum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
reaction rate with NADPH is approximately twice of that with NADH
Pisum sativum
NADPH
reaction rate with NADPH is approximately twice of that with NADH
Pisum sativum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AgNO3
0.009 mM, 66% inhibition
Pisum sativum
arsenite
0.15 mM, complete inhibition
Pisum sativum
CdSO4
0.005 mM, 91% inhibition
Pisum sativum
CuSO4
0.015 mM, complete inhibition
Pisum sativum
HgCl2
0.002 mM, 92% inhibition
Pisum sativum
iodoacetate
0.054 mM, 57% inhibition
Pisum sativum
p-chloromercuribenzoate
0.054 mM, 88% inhibition
Pisum sativum
ZnSO4
0.003 mM, 82% inhibition
Pisum sativum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
NADPH
-
Pisum sativum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pisum sativum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Hordeum vulgare
-
root
-
Pisum sativum
-
root
-
Zea mays
-
seed
-
Avena sativa
-
seed
-
Hordeum vulgare
-
seed
-
Pisum sativum
-
seed
-
Triticum aestivum
-
seed
-
Vicia faba
-
seed
-
Zea mays
-
shoot
-
Hordeum vulgare
-
shoot
-
Pisum sativum
-
shoot
-
Triticum aestivum
-
shoot
-
Vicia faba
-
shoot
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pisum sativum
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, 8 weeks, stable
Vicia faba
-15°C, 8 weeks, stable
Triticum aestivum
-15°C, 8 weeks, stable
Hordeum vulgare
-15°C, 8 weeks, stable
Pisum sativum
-15°C, 8 weeks, stable
Zea mays
-15°C, 8 weeks, stable
Avena sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD(P)H + protein disulfide
-
437710
Vicia faba
NAD(P)+ + protein dithiol
-
437710
Vicia faba
?
NAD(P)H + protein disulfide
-
437710
Triticum aestivum
NAD(P)+ + protein dithiol
-
437710
Triticum aestivum
?
NAD(P)H + protein disulfide
-
437710
Hordeum vulgare
NAD(P)+ + protein dithiol
-
437710
Hordeum vulgare
?
NAD(P)H + protein disulfide
-
437710
Zea mays
NAD(P)+ + protein dithiol
-
437710
Zea mays
?
NAD(P)H + protein disulfide
-
437710
Avena sativa
NAD(P)+ + protein dithiol
-
437710
Avena sativa
?
NAD(P)H + protein disulfide
reaction rate with NADPH is approximately twice that with NADH
437710
Pisum sativum
NAD(P)+ + protein dithiol
-
437710
Pisum sativum
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
10 min, stable
Pisum sativum
70
-
10 min, complete inactivation
Pisum sativum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
7.4
-
Pisum sativum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
7.8
pH 6.2: about 50% of activity maximum, pH 7.8: about 20% of activity maximum
Pisum sativum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
-
5°C or 22°C, irreversible inactivation
Pisum sativum
Other publictions for EC 1.8.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742471
Lim
Redox regulation of SurR by p ...
Thermococcus onnurineus
Extremophiles
21
491-498
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741927
Yenugudhati
Structural and biochemical ch ...
Methanosarcina acetivorans, Methanosarcina acetivorans DSM 2834
Biochemistry
55
313-321
2016
-
-
1
1
-
-
-
3
-
-
1
-
-
6
-
-
-
-
-
-
-
-
8
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743076
Choi
Dimethyl sulfoxide reduction ...
Thermococcus onnurineus
J. Microbiol.
54
31-38
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742467
Pedone
Functional and structural cha ...
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
Extremophiles
18
723-731
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
725973
Hagiwara
Structural basis of an ERAD pa ...
Mus musculus
Mol. Cell
41
432-444
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
745744
Scirè
High hydrostatic pressure-ind ...
Pyrococcus furiosus
Mol. Biosyst.
6
2015-2022
2010
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687756
Jeong
ERp16, an endoplasmic reticulu ...
Homo sapiens
J. Biol. Chem.
283
25557-25566
2008
-
-
1
-
-
-
-
-
1
-
2
-
-
2
-
-
1
-
-
1
-
-
1
1
-
-
-
-
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-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670797
Garg
Characterization of Mycobacter ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis Rv3219
Protein Expr. Purif.
52
422-432
2007
-
-
1
-
-
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
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-
2
-
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-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687393
Lessner
The archaeon Methanosarcina ac ...
Methanosarcina acetivorans
J. Bacteriol.
189
7475-7484
2007
-
-
1
-
-
-
-
-
-
1
-
1
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
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-
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-
1
1
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-
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-
-
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-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722206
Kuroita
Functional similarities of a t ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
Extremophiles
11
85-94
2007
-
-
1
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
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1
-
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1
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-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
671296
Tomazzolli
A fluorescence-based assay for ...
Homo sapiens
Anal. Biochem.
350
105-112
2006
-
-
-
-
-
-
-
-
-
-
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-
1
-
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-
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-
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-
-
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-
-
-
-
673557
Ladenstein
Protein disulfides and protein ...
Pyrococcus furiosus, Pyrococcus horikoshii, Saccharolobus solfataricus, Thermococcus kodakarensis, Thermotoga maritima
FEBS J.
273
4170-4185
2006
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677156
Maeda
Structural basis for target pr ...
Hordeum vulgare
Structure
14
1701-1710
2006
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
-
-
-
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-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657677
Poole
Bacterial defenses against oxi ...
Salmonella enterica subsp. enterica serovar Typhimurium
Arch. Biochem. Biophys.
433
240-254
2005
-
-
-
1
1
-
1
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
5
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
745638
Pedone
Temperature-, SDS-, and pH-in ...
Pyrococcus furiosus
J. Proteome Res.
4
1972-1980
2005
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
1
-
-
-
3
-
1
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
3
-
1
-
3
-
-
-
-
-
-
-
659424
Argyrou
Characterization of a new memb ...
Mycobacterium tuberculosis
J. Biol. Chem.
279
52694-52702
2004
-
-
1
1
-
-
2
11
-
-
-
1
-
4
-
-
1
1
-
-
-
-
6
1
1
-
-
12
1
-
-
2
2
-
-
-
-
1
2
1
-
-
-
2
2
11
-
-
-
1
-
-
-
1
-
-
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