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Literature summary for 1.8.1.7 extracted from

  • Wang, Y.; Wang, Q.; Hou, Y.
    A new cold-adapted and salt-tolerant glutathione reductase from antarctic psychrophilic bacterium Psychrobacter sp. and its resistance to oxidation (2020), Int. J. Mol. Sci., 21, 420 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.7

Cloned(Commentary)

Cloned (Comment) Organism
overexpression into Escherichia coli Psychrobacter sp. ANT206

Inhibitors

Inhibitors Comment Organism Structure
BaCl2 0.25 mM, 62.7% loss of activity Psychrobacter sp. ANT206
CaCl2 0.25 mM, 9.1% loss of activity Psychrobacter sp. ANT206
CdCl2 0.25 mM, 80.9% loss of activity Psychrobacter sp. ANT206
CoCl2 0.25 mM, 72.7% loss of activity Psychrobacter sp. ANT206
CrCl2 0.25 mM, 52.7% loss of activity Psychrobacter sp. ANT206
CuCl2 0.25 mM, 79.7% loss of activity Psychrobacter sp. ANT206
EDTA 0.25 mM, 71.8% loss of activity Psychrobacter sp. ANT206
FeCl2 0.25 mM, 55.5% loss of activity Psychrobacter sp. ANT206
HgCl2 0.25 mM, 89.1% loss of activity Psychrobacter sp. ANT206
KCl 0.25 mM, 9.7% loss of activity Psychrobacter sp. ANT206
Pb(NO3)2 0.25 mM, complete loss of activity Psychrobacter sp. ANT206
ZnCl2 0.25 mM, 66.4% loss of activity Psychrobacter sp. ANT206

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01695
-
 NADPH pH 7.5, 25°C Psychrobacter sp. ANT206
0.06896
-
 glutathione disulfide pH 7.5, 25°C Psychrobacter sp. ANT206

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 0.25, 1.1fold activation Psychrobacter sp. ANT206
NaCl most active in 1.0 M NaCl and 62.5% of its full activity remains in 3.0 M NaCl, demonstrating its high salt tolerance Psychrobacter sp. ANT206

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53500
-
 SDS-PAGE Psychrobacter sp. ANT206

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH + H+ Psychrobacter sp. ANT206
-
 2 glutathione + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Psychrobacter sp. ANT206
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Psychrobacter sp. ANT206

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
168.8
-
 pH 7.5, 25°C Psychrobacter sp. ANT206

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
 Psychrobacter sp. ANT206 2 glutathione + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 53500, SDS-PAGE Psychrobacter sp. ANT206

Synonyms

Synonyms Comment Organism
glutathione reductase
-
 Psychrobacter sp. ANT206
psgr
-
 Psychrobacter sp. ANT206

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Psychrobacter sp. ANT206

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 45 5°C: about 50% of maximal activity, 45°C: about 55% of maximal activity Psychrobacter sp. ANT206

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 60 min, about 15% loss of activity Psychrobacter sp. ANT206
30
-
 60 min, about 20% loss of activity Psychrobacter sp. ANT206
35
-
 60 min, about 40% loss of activity Psychrobacter sp. ANT206
40
-
 60 min, about 45% loss of activity Psychrobacter sp. ANT206
45
-
 60 min, about 55% loss of activity Psychrobacter sp. ANT206
50
-
 60 min, about 60% loss of activity Psychrobacter sp. ANT206
55
-
 60 min, complete loss of activity Psychrobacter sp. ANT206

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
95.78
-
 NADPH pH 7.5, 25°C Psychrobacter sp. ANT206
95.78
-
 glutathione disulfide pH 7.5, 25°C Psychrobacter sp. ANT206

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Psychrobacter sp. ANT206

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: about 50% of maximal activity, pH 8.5: about 70% of maximal activity Psychrobacter sp. ANT206

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 30 min, about 60% loss of activity Psychrobacter sp. ANT206
5
-
 30 min, about 50% loss of activity Psychrobacter sp. ANT206
5.5
-
 30 min, about 40% loss of activity Psychrobacter sp. ANT206
6
-
 30 min, about 35% loss of activity Psychrobacter sp. ANT206
6.5
-
 30 min, about 30% loss of activity Psychrobacter sp. ANT206
7
-
 30 min, about 15% loss of activity Psychrobacter sp. ANT206
7.5
-
 30 min, about less than 5% loss of activity Psychrobacter sp. ANT206
8
-
 30 min, about 15% loss of activity Psychrobacter sp. ANT206
8.5
-
 30 min, about 30% loss of activity Psychrobacter sp. ANT206

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme possesses the flavin adenine dinucleotide binding motif Psychrobacter sp. ANT206

General Information

General Information Comment Organism
physiological function the enzyme provides protection against peroxide (H2O2)-induced oxidative stress in recombinant cells Psychrobacter sp. ANT206