Any feedback?
Literature summary for 1.8.1.7 extracted from
- The unique glutathione reductase from Xanthomonas campestris: gene expression and enzyme characterization (2005), Biochem. Biophys. Res. Commun., 331, 1324-1330.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Xanthomonas campestris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0526 | - |
NADPH | - |
Xanthomonas campestris |  |
| 0.0555 | - |
NADH | - |
Xanthomonas campestris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Xanthomonas campestris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas campestris | Q56S00 | Xanthomonas campestris pv. phaseoli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography | Xanthomonas campestris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.039 | - |
using NADH as electron donor | Xanthomonas campestris |
| 0.045 | - |
using NADPH as electron donor | Xanthomonas campestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADH + H+ | - |
Xanthomonas campestris | 2 glutathione + NAD+ | - |
? | |
| glutathione disulfide + NADH + H+ | - |
Xanthomonas campestris | glutathione + NAD+ | - |
? | |
| glutathione disulfide + NADPH + H+ | - |
Xanthomonas campestris | glutathione + NADP+ | - |
r | |
| additional information | the enzyme has the ability to utilize both NADPH and NADH as electron donors | Xanthomonas campestris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, gel filtration | Xanthomonas campestris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione reductase | - |
Xanthomonas campestris |
| Gor | - |
Xanthomonas campestris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32.5 | - |
NADH | - |
Xanthomonas campestris | |
| 37.5 | - |
NADPH | - |
Xanthomonas campestris | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
