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Literature summary for 1.8.1.7 extracted from

  • Rendon, J.L.; del Arenal, I.P.; Guevara-Flores, A.; Uribe, A.; Plancarte, A.; Mendoza-Hernandez, G.
    Purification, characterization and kinetic properties of the multifunctional thioredoxin-glutathione reductase from Taenia crassiceps metacestode (cysticerci) (2004), Mol. Biochem. Parasitol., 133, 61-69.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
auranofin complete inhibition of both enzyme reductase activities at 10 nM Taenia crassiceps

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, hysteretic behaviour Taenia crassiceps
0.003
-
NADPH pH 7.8, 25°C Taenia crassiceps
0.015
-
glutathione disulfide pH 7.8, 25°C Taenia crassiceps

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Taenia crassiceps
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
2 * 65000, SDS-PAGE Taenia crassiceps
136000
-
gel filtration Taenia crassiceps

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH Taenia crassiceps
-
glutathione + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Taenia crassiceps
-
parasite isolated from mice, enzyme is a multifunctional thioredoxin-glutathione reductase TGR
-

Purification (Commentary)

Purification (Comment) Organism
647fold to homogeneity from metacystodes by ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography, and gel filtration Taenia crassiceps

Reaction

Reaction Comment Organism Reaction ID
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ the multifunctional enzyme possesses both thioredoxin reductase activity, EC 1.8.1.9, glutathione reductase activity, EC 1.8.1.7, and glutaredoxin activity Taenia crassiceps

Source Tissue

Source Tissue Comment Organism Textmining
metacestode i.e. cysticeri, recovered from peritoneal cavity of 6-8 months before inoculated female Balb/c mice Taenia crassiceps
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
purified enzyme, substrate glutathione disulfide Taenia crassiceps

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH
-
Taenia crassiceps glutathione + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 65000, SDS-PAGE Taenia crassiceps

Synonyms

Synonyms Comment Organism
multifunctional thioredoxin-glutathione reductase
-
Taenia crassiceps
TGR
-
Taenia crassiceps

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Taenia crassiceps

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Taenia crassiceps

Cofactor

Cofactor Comment Organism Structure
FAD 1.2 mol per mol of enzyme subunit bound Taenia crassiceps
NADPH
-
Taenia crassiceps

pI Value

Organism Comment pI Value Maximum pI Value
Taenia crassiceps isoelectric focusing
-
5.2