Cloned (Comment) | Organism |
---|---|
construction of a genetic library, DNA and amino acid sequence determination and analysis of the enzyme encoding gene, expression in Escherichia coli | Physarum polycephalum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0286 | - |
NADPH | - |
Physarum polycephalum | |
0.04 | - |
glutathione disulfide | - |
Physarum polycephalum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49300 | - |
2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry | Physarum polycephalum |
50000 | - |
2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry | Physarum polycephalum |
99200 | - |
velocity sedimentation analysis | Physarum polycephalum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH | Physarum polycephalum | - |
glutathione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | Q9NL96 | strain ATCC 24466 and 24467 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from microplasmodia, 968fold in a multistep procedure | Physarum polycephalum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
microplasmodium | - |
Physarum polycephalum | - |
additional information | stage-specific expression of the enzyme in life-cycle stages with different oxidation-reduction levels, glutathione levels, overview | Physarum polycephalum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
484 | - |
purified native enzyme | Physarum polycephalum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH | - |
Physarum polycephalum | glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry | Physarum polycephalum |
Synonyms | Comment | Organism |
---|---|---|
glutathione reductase | - |
Physarum polycephalum |
GR | - |
Physarum polycephalum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Physarum polycephalum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Physarum polycephalum |