Literature summary for 1.8.1.7 extracted from

Stoll, V.S.; Simpson, S.J.; Krauth-Siegel, R.L.; Walsh, C.T.; Pai, E.F.
Glutathione reductase turned into trypanothione reductase: structural analysis of an engineered change in substrate specificity (1997), Biochemistry, 36, 6437-6447.

Search for more literature for 1.8.1.7

Activating Compound

Activating Compound	Comment	Organism	Structure
cysteine	redox active disulfide at the catalytic site	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
mutant enzyme glutathione reductase-trypanothine reductase, expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
substrate-binding and structure analysis	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A34E/R37W	glutathione reductase mutant, activity switches to trypanothione reductase, termed GRTR, 700fold more activity with trypanothione than with glutathione	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.065	-	GSSG	-	Homo sapiens
0.5	-	trypanothione	recombinant glutathione-trypanothione reductase-like enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00390	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant mutant from Escherichia coli	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+	member of pyridine-nucleotide disulfide oxidoreductase family of flavoenzymes	Homo sapiens	a
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+	recombinant glutathione-trypanothione reductase-like enzyme, substrate binding, three dimensional structure, complex formation	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GSSG + NADPH	-	Homo sapiens	glutathione + NADP+	-	?
trypanothione + NADPH	recombinant glutathione-trypanothione reductase-like enzyme	Homo sapiens	reduced trypanothione + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
GR	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
20.8	-	trypanothione	recombinant glutathione-trypanothione reductase-like enzyme	Homo sapiens
210	-	GSSG	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	recombinant glutathione-trypanothione reductase-like enzyme	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Homo sapiens
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)