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Literature summary for 1.8.1.7 extracted from
- Occurrence of two isoforms of glutathione reductase in the green alga Chlamydomonas reinhardtii (1993), Planta, 190, 199-205.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | slightly | Chlamydomonas reinhardtii |  |
| Co2+ | slightly | Chlamydomonas reinhardtii | |
| Cu2+ | - |
Chlamydomonas reinhardtii | |
| Hg2+ | - |
Chlamydomonas reinhardtii | |
| Mn2+ | slightly | Chlamydomonas reinhardtii | |
| phenyl mercuric acetate | total inhibition | Chlamydomonas reinhardtii | |
| Phenylglyoxal | slightly | Chlamydomonas reinhardtii | |
| Zn2+ | - |
Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
NADPH | isozyme GR-1 | Chlamydomonas reinhardtii | |
| 0.028 | - |
NADPH | isozyme GR-2 | Chlamydomonas reinhardtii | |
| 0.05 | - |
GSSG | isozmes GR-1 and GR-2 | Chlamydomonas reinhardtii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | isoform dependent | Chlamydomonas reinhardtii | 9507 | - |
| cytoplasm | isoform dependent | Chlamydomonas reinhardtii | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
2 * 66000, SDS-PAGE | Chlamydomonas reinhardtii |
| 127000 | - |
gel filtration | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
2 isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42 | 60 | purified enzyme | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Chlamydomonas reinhardtii | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 66000, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GR | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Chlamydomonas reinhardtii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Chlamydomonas reinhardtii | |
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