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Literature summary for 1.8.1.7 extracted from
- Mouse-liver glutathione reductase. Purification, kinetics, and regulation (1979), Eur. J. Biochem., 98, 487-499.
General Stability
| General Stability | Organism |
|---|---|
| complete inactivation after incubation with NADH, reversible by addition of GSSG | Mus musculus |
| complete inactivation after incubation with NADPH, reversible by addition of GSSG | Mus musculus |
| dichloroindophenol protects against thermal inactivation at 80°C | Mus musculus |
| FAD, no stabilization against thermal inactivation at 80°C | Mus musculus |
| glutathione protects against thermal inactivation at 80°C | Mus musculus |
| GSSG protects against thermal inactivation at 80°C | Mus musculus |
| NADP+, stabilizes against thermal inactivation at 80°C | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | product inhibition | Mus musculus |  |
| NADH | at concentration above 0.3 mM | Mus musculus | |
| NADP+ | competitive against NADPH | Mus musculus | |
| NADPH | at concentration above 0.3 mM | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0059 | - |
NADPH | - |
Mus musculus | |
| 0.107 | - |
GSSG | - |
Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Mus musculus |
| 105000 | - |
gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 158 | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, pH 7.0, 1 mM EDTA, 1 mM dithiothreitol, 0.020 mM FAD, 5 mM NADP+ | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Mus musculus | glutathione + NADP+ | - |
? | |
| additional information | branched mechanism | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
8 h, stable | Mus musculus |
| 75 | - |
10 min, stable | Mus musculus |
| 80 | - |
NADP+, GSSG, glutathione and dichloroindophenol protect against thermal inactivation at 80°C | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Mus musculus | |
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