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Literature summary for 1.8.1.7 extracted from

  • Rendon, J.L.; Calcagno, M.; Mendoza-Hernandez, G.; Ondarza, R.N.
    Purification, properties, and oligomeric structure of glutathione reductase from the cyanobacterium Spirulina maxima (1986), Arch. Biochem. Biophys., 248, 215-223.
    View publication on PubMed

General Stability

General Stability Organism
repeated freezing and thawing, no effect Limnospira maxima
urea, activation from 0.4-0.6 M, inactivation at higher concentration Limnospira maxima

Inhibitors

Inhibitors Comment Organism Structure
Urea activation: 0.4-0.6 M, inactivation at higher concentration Limnospira maxima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.121
-
GSSG
-
Limnospira maxima
3.3
-
mixed disulfide of CoA and glutathione
-
Limnospira maxima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 47000, SDS-PAGE, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima
47000
-
4 * 47000, SDS-PAGE, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima
99800
-
Spirulina maxima, pore gradient gel electrophoresis, dimer, enzyme exists predominantly as tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima
177000
-
sedimentation equilibrium, tetramer, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima

Organism

Organism UniProt Comment Textmining
Limnospira maxima
-
cyanobacterium
-

Purification (Commentary)

Purification (Comment) Organism
-
Limnospira maxima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
238
-
-
Limnospira maxima

Storage Stability

Storage Stability Organism
-20°C, 1 year Limnospira maxima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + NADP+ mixed disulfide of CoA and glutathione Limnospira maxima glutathione disulfide + NADPH + H+
-
r
GSSG + NADPH mixed disulfide between coenzyme A and glutathione Limnospira maxima glutathione + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima
tetramer 4 * 47000, SDS-PAGE, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Limnospira maxima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
10 min, pH 7.0, stable Limnospira maxima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Limnospira maxima

Cofactor

Cofactor Comment Organism Structure
FAD FAD enzyme Limnospira maxima
flavin 1.06 mol of flavin per mol of subunit Limnospira maxima
NADPH absolute specificity for NADPH Limnospira maxima