BRENDA - Reference to 1.8.1.6; Id = 743682

recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Homo sapiens

C43S/C46S

site-directed mutagenesis, inactive mutant

Homo sapiens

C46S

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

Homo sapiens

additional information

knockdown and overexpression of TRP14 affect total L-cystine reduction capacity and S-nitrosoprotein levels in crude HEK-293 cell lysate

Homo sapiens

additional information

-

additional information

Michaelis-Menten kinetics

Homo sapiens

0.0008

-

L-cystine

pH 7.5, 22°C, wild-type enzyme TRP14

Homo sapiens

cytosol

-

Homo sapiens

5829

-

L-cystine + NADH + H+

Homo sapiens

-

2 L-cysteine + NAD+

-

?

additional information

Homo sapiens

enzyme TRP14 not being able to reduce any classical protein disulfide substrates of thioredoxin 1, Trx1. Trx1-mediated L-cystine reduction is inefficient in the presence of other preferred Trx1 substrates whereas, conversely, TRP14 seems to be a more dedicated L-cystine reductase

?

-

Homo sapiens

Q9BRA2

-

recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)

Homo sapiens

A-431 cell

high enzyme expression level

Homo sapiens

-

HEK-293 cell

-

Homo sapiens

-

L-cystine + NADH + H+

-

743682

Homo sapiens

2 L-cysteine + NAD+

-

?

additional information

enzyme TRP14 not being able to reduce any classical protein disulfide substrates of thioredoxin 1, Trx1. Trx1-mediated L-cystine reduction is inefficient in the presence of other preferred Trx1 substrates whereas, conversely, TRP14 seems to be a more dedicated L-cystine reductase

743682

Homo sapiens

?

-

additional information

enzyme TRP14 has S-denitrosylase activity performing GSNO reduction with realease of NO, it is also active with L-CysSNO

743682

Homo sapiens

?

-

L-cystine reductase

-

Homo sapiens

thioredoxin domain containing 17

-

Homo sapiens

thioredoxin-like 5

-

Homo sapiens

TRP14

-

Homo sapiens

TXNDC17

-

Homo sapiens

TXNL5

-

Homo sapiens

22

-

assay at room temperature

Homo sapiens

30.3

-

L-cystine

pH 7.5, 22°C, wild-type enzyme TRP14

Homo sapiens

7.5

-

assay at

Homo sapiens

NADH

-

Homo sapiens

recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Homo sapiens

NADH

-

Homo sapiens

C43S/C46S

site-directed mutagenesis, inactive mutant

Homo sapiens

C46S

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

Homo sapiens

additional information

knockdown and overexpression of TRP14 affect total L-cystine reduction capacity and S-nitrosoprotein levels in crude HEK-293 cell lysate

Homo sapiens

additional information

-

additional information

Michaelis-Menten kinetics

Homo sapiens

0.0008

-

L-cystine

pH 7.5, 22°C, wild-type enzyme TRP14

Homo sapiens

cytosol

-

Homo sapiens

5829

-

L-cystine + NADH + H+

Homo sapiens

-

2 L-cysteine + NAD+

-

?

additional information

Homo sapiens

enzyme TRP14 not being able to reduce any classical protein disulfide substrates of thioredoxin 1, Trx1. Trx1-mediated L-cystine reduction is inefficient in the presence of other preferred Trx1 substrates whereas, conversely, TRP14 seems to be a more dedicated L-cystine reductase

?

-

recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)

Homo sapiens

A-431 cell

high enzyme expression level

Homo sapiens

-

HEK-293 cell

-

Homo sapiens

-

L-cystine + NADH + H+

-

743682

Homo sapiens

2 L-cysteine + NAD+

-

?

additional information

enzyme TRP14 not being able to reduce any classical protein disulfide substrates of thioredoxin 1, Trx1. Trx1-mediated L-cystine reduction is inefficient in the presence of other preferred Trx1 substrates whereas, conversely, TRP14 seems to be a more dedicated L-cystine reductase

743682

Homo sapiens

?

-

additional information

enzyme TRP14 has S-denitrosylase activity performing GSNO reduction with realease of NO, it is also active with L-CysSNO

743682

Homo sapiens

?

-

22

-

assay at room temperature

Homo sapiens

30.3

-

L-cystine

pH 7.5, 22°C, wild-type enzyme TRP14

Homo sapiens

7.5

-

assay at

Homo sapiens

evolution

the enzyme belongs to the member of the thioredoxin (Trx)-fold protein family. The enzyme lacks activity with classical thioredoxin, Trx1, substrates. Human enzyme TRP14 has high enzymatic activity in reduction of L-cystine, where the catalytic efficiency coupled to Trx reductase 1 (TrxR1) using NADPH is fivefold higher than the activity of enzyme Trx1 alone. Trx1-mediated L-cystine reduction is inefficient in the presence of other preferred Trx1 substrates whereas, conversely, TRP14 seems to be a more dedicated L-cystine reductase

Homo sapiens

additional information

the disulfide/dithiol motif formed by Cys43 and Cys46 is the active site of TRP14

Homo sapiens

evolution

the enzyme belongs to the member of the thioredoxin (Trx)-fold protein family. The enzyme lacks activity with classical thioredoxin, Trx1, substrates. Human enzyme TRP14 has high enzymatic activity in reduction of L-cystine, where the catalytic efficiency coupled to Trx reductase 1 (TrxR1) using NADPH is fivefold higher than the activity of enzyme Trx1 alone. Trx1-mediated L-cystine reduction is inefficient in the presence of other preferred Trx1 substrates whereas, conversely, TRP14 seems to be a more dedicated L-cystine reductase

Homo sapiens

additional information

the disulfide/dithiol motif formed by Cys43 and Cys46 is the active site of TRP14

Homo sapiens

37875

-

L-cystine

pH 7.5, 22°C, wild-type enzyme TRP14

Homo sapiens

37875

-

L-cystine

pH 7.5, 22°C, wild-type enzyme TRP14

Homo sapiens

743682

Pader

Thioredoxin-related protein o ...

Homo sapiens

Proc. Natl. Acad. Sci. USA

111

6964-6969

2014

-

1

-

3

-

2

1

-

2

-

2

-

1

-

2

-

3

-

6

1

-

1

-

1

-

1

-

3

-

2

1

-

2

-

1

-

2

-

3

-

1

-

1

-

2

-

1

673491

Ondarza

The effects by neuroleptics, a ...

Acanthamoeba polyphaga, Naegleria fowleri

Exp. Parasitol.

115

41-47

2007

4

-

11

-

5

-

2

-

2

-

4

-

11

-

2

-

667725

Kim

Properties of the cysteine res ...

Ulva intestinalis

Biochemistry

45

5010-5018

2006

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

440354

Maresca

Cystine reductase in the dimor ...

Histoplasma capsulatum

J. Bacteriol.

135

987-992

1978

-

1

-

1

-

3

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

440356

Carroll

alpha-Substituted cystines as ...

Saccharomyces cerevisiae

Biochim. Biophys. Acta

198

601-603

1970

-

2

-

1

-

1

-

1

-

1

-

2

-

1

-

440355

Thibert

-

Simultaneous determination of ...

Saccharomyces cerevisiae

Mikrochim. Acta

3

615-624

1969

-

1

-

1

-

1

-

1

-

1

-

440353

Romano

Cystine reductase of pea seeds ...

Candida albicans, Pisum sativum, Saccharomyces cerevisiae

J. Biol. Chem.

208

409-416

1954

-

5

-

3

-

3

-

3

-

3

-

3

-

3

-

Thioredoxin-related protein of 14 kDa is an efficient L-cystine reductase and S-denitrosylase

Data extracted from this reference: