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Literature summary for 1.8.1.5 extracted from

  • Nocek, B.; Jang, S.B.; Jeong, M.S.; Clark, D.D.; Ensign, S.A.; Peters, J.W.
    Structural basis for CO2 fixation by a novel member of the disulfide oxidoreductase family of enzymes, 2-ketopropyl-coenzyme M oxidoreductase/carboxylase (2002), Biochemistry, 41, 12907-12913.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme free or bound to substrate 2-ketopropyl-CoM, X-ray structure determination and analysis at 1.6-3.5 A resolution, multiple isomorphous replacement and anomalous scattering using four weak heavy atom derivatives Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH Xanthobacter autotrophicus terminal enzyme in the metabolic pathway converting propylene to acetoacetate 2-mercaptoethanesulfonate + acetoacetate + NADP+
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r

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus Q56839
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Reaction

Reaction Comment Organism Reaction ID
2-mercaptoethanesulfonate + acetoacetate + NADP+ = 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+ detailed reaction mechanism in a catalytic cycle, active site structure Xanthobacter autotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH terminal enzyme in the metabolic pathway converting propylene to acetoacetate Xanthobacter autotrophicus 2-mercaptoethanesulfonate + acetoacetate + NADP+
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r
2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH i.e. 2-ketopropyl-CoM, comparison of enzyme structure with and without bound substrate, binding of 2-ketopropyl-coenzyme M induces a conformational change resulting in collapse of the substrate access channel, substrate binding site structure analysis Xanthobacter autotrophicus 2-mercaptoethanesulfonate + acetoacetate + NADP+
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r

Subunits

Subunits Comment Organism
dimer crystal structure Xanthobacter autotrophicus

Synonyms

Synonyms Comment Organism
2-ketopropyl-coenzyme M oxidoreductase/carboxylase
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Xanthobacter autotrophicus
2-KPCC
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Xanthobacter autotrophicus
More enzyme belongs to the disulfide oxidoreductase family of enzymes DSOR Xanthobacter autotrophicus
NADPH:2-ketopropyl-CoM oxidoreductase/carboxylase
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Xanthobacter autotrophicus

Cofactor

Cofactor Comment Organism Structure
FAD binding domain structure analysis Xanthobacter autotrophicus
NADP+ binding domain structure analysis Xanthobacter autotrophicus
NADPH binding domain structure analysis Xanthobacter autotrophicus