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Literature summary for 1.8.1.4 extracted from

  • Kim, H.
    Characterization of human dihydrolipoamide dehydrogenase mutant showing significantly decreased catalytic efficiency (2019), J. Korean Chem. Soc., 63, 134-137 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
L99A the mutation deteriorates the catalytic power of the enzyme substantially Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
NAD+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens
0.31
-
dihydrolipoamide mutant enzyme L99A, at pH 8.0 and 37°C Homo sapiens
0.64
-
dihydrolipoamide wild type enzyme, at pH 8.0 and 37°C Homo sapiens
1.3
-
NAD+ mutant enzyme L99A, at pH 8.0 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09622
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA His-Bind resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
Homo sapiens lipoamide + NADH + H+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 50216, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
Homo sapiens
dihydrolipoamide:NAD+ oxidoreductase
-
Homo sapiens
E3
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.2
-
NAD+ mutant enzyme L99A, at pH 8.0 and 37°C Homo sapiens
16.2
-
dihydrolipoamide mutant enzyme L99A, at pH 8.0 and 37°C Homo sapiens
899
-
NAD+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens
899
-
dihydrolipoamide wild type enzyme, at pH 8.0 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12
-
NAD+ mutant enzyme L99A, at pH 8.0 and 37°C Homo sapiens
52
-
dihydrolipoamide mutant enzyme L99A, at pH 8.0 and 37°C Homo sapiens
1405
-
dihydrolipoamide wild type enzyme, at pH 8.0 and 37°C Homo sapiens
4732
-
NAD+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens