Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L99A | the mutation deteriorates the catalytic power of the enzyme substantially | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
NAD+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
0.31 | - |
dihydrolipoamide | mutant enzyme L99A, at pH 8.0 and 37°C | Homo sapiens | |
0.64 | - |
dihydrolipoamide | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
1.3 | - |
NAD+ | mutant enzyme L99A, at pH 8.0 and 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09622 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA His-Bind resin column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50216, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Homo sapiens |
dihydrolipoamide:NAD+ oxidoreductase | - |
Homo sapiens |
E3 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.2 | - |
NAD+ | mutant enzyme L99A, at pH 8.0 and 37°C | Homo sapiens | |
16.2 | - |
dihydrolipoamide | mutant enzyme L99A, at pH 8.0 and 37°C | Homo sapiens | |
899 | - |
NAD+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
899 | - |
dihydrolipoamide | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
NAD+ | mutant enzyme L99A, at pH 8.0 and 37°C | Homo sapiens | |
52 | - |
dihydrolipoamide | mutant enzyme L99A, at pH 8.0 and 37°C | Homo sapiens | |
1405 | - |
dihydrolipoamide | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
4732 | - |
NAD+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens |