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Literature summary for 1.8.1.4 extracted from

  • Chiranjivi, A.K.; Dubey, V.K.
    Dihydrolipoamide dehydrogenase from Leishmania donovani New insights through biochemical characterization (2018), Int. J. Biol. Macromol., 112, 1241-1247 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami cells Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17123
-
NAD+ enzyme variant 1, at pH 7.5 and 37°C Leishmania donovani
0.266
-
NAD+ enzyme variant 2, at pH 7.5 and 37°C Leishmania donovani
0.8299
-
dihydrolipoamide enzyme variant 2, at pH 7.5 and 37°C Leishmania donovani
2.7
-
dihydrolipoamide enzyme variant 1, at pH 7.5 and 37°C Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-
Leishmania donovani BHU1081
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
Leishmania donovani lipoamide + NADH + H+
-
r
dihydrolipoamide + NAD+
-
Leishmania donovani BHU1081 lipoamide + NADH + H+
-
r
additional information the enzyme also shows diaphorase activity with 2,6-dichlorophenol indophenol and thiazolyl blue tetrazolium bromide Leishmania donovani ?
-
-
additional information the enzyme also shows diaphorase activity with 2,6-dichlorophenol indophenol and thiazolyl blue tetrazolium bromide Leishmania donovani BHU1081 ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 50600, variant 2, SDS-PAGE Leishmania donovani
homodimer 2 * 56300, variant 1, SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
Leishmania donovani
DLDH
-
Leishmania donovani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
temperature optimum of the reverse reaction for variant 2 Leishmania donovani
25 37 temperature optimum of the reverse reaction for variant 1 Leishmania donovani
50
-
temperature optimum of the forward reaction for variants 1 and 2 Leishmania donovani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0027
-
NAD+ enzyme variant 2, at pH 7.5 and 37°C Leishmania donovani
0.0045
-
NAD+ enzyme variant 1, at pH 7.5 and 37°C Leishmania donovani
0.012
-
dihydrolipoamide enzyme variant 2, at pH 7.5 and 37°C Leishmania donovani
0.122
-
dihydrolipoamide enzyme variant 1, at pH 7.5 and 37°C Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 pH optimum of the reverse reaction for variant 1 Leishmania donovani
7.5
-
pH optimum of the forward reaction for variant 2 Leishmania donovani
7.5 8 pH optimum of the forward reaction for variant 1 Leishmania donovani
9
-
pH optimum of the reverse reaction for variant 2 Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Leishmania donovani
NAD+
-
Leishmania donovani