Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta-gami cells | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17123 | - |
NAD+ | enzyme variant 1, at pH 7.5 and 37°C | Leishmania donovani | |
0.266 | - |
NAD+ | enzyme variant 2, at pH 7.5 and 37°C | Leishmania donovani | |
0.8299 | - |
dihydrolipoamide | enzyme variant 2, at pH 7.5 and 37°C | Leishmania donovani | |
2.7 | - |
dihydrolipoamide | enzyme variant 1, at pH 7.5 and 37°C | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Leishmania donovani BHU1081 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Leishmania donovani | lipoamide + NADH + H+ | - |
r | |
dihydrolipoamide + NAD+ | - |
Leishmania donovani BHU1081 | lipoamide + NADH + H+ | - |
r | |
additional information | the enzyme also shows diaphorase activity with 2,6-dichlorophenol indophenol and thiazolyl blue tetrazolium bromide | Leishmania donovani | ? | - |
- |
|
additional information | the enzyme also shows diaphorase activity with 2,6-dichlorophenol indophenol and thiazolyl blue tetrazolium bromide | Leishmania donovani BHU1081 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50600, variant 2, SDS-PAGE | Leishmania donovani |
homodimer | 2 * 56300, variant 1, SDS-PAGE | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Leishmania donovani |
DLDH | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
temperature optimum of the reverse reaction for variant 2 | Leishmania donovani |
25 | 37 | temperature optimum of the reverse reaction for variant 1 | Leishmania donovani |
50 | - |
temperature optimum of the forward reaction for variants 1 and 2 | Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
NAD+ | enzyme variant 2, at pH 7.5 and 37°C | Leishmania donovani | |
0.0045 | - |
NAD+ | enzyme variant 1, at pH 7.5 and 37°C | Leishmania donovani | |
0.012 | - |
dihydrolipoamide | enzyme variant 2, at pH 7.5 and 37°C | Leishmania donovani | |
0.122 | - |
dihydrolipoamide | enzyme variant 1, at pH 7.5 and 37°C | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | pH optimum of the reverse reaction for variant 1 | Leishmania donovani |
7.5 | - |
pH optimum of the forward reaction for variant 2 | Leishmania donovani |
7.5 | 8 | pH optimum of the forward reaction for variant 1 | Leishmania donovani |
9 | - |
pH optimum of the reverse reaction for variant 2 | Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Leishmania donovani | |
NAD+ | - |
Leishmania donovani |