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Literature summary for 1.8.1.4 extracted from
- Characterization of site-specific mutations affecting the catalytic efficiency of human dihydrolipoamide dehydrogenase toward NAD+ (2015), J. Korean Chem. Soc., 59, 183-187 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene dld, located on chromosome 7, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain XL-1 Blue | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| P156A | site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme | Homo sapiens |
| P303A | site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
NAD+ | wild-type enzyme, pH 8.0, 37°C | Homo sapiens |  |
| 0.24 | - |
NAD+ | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
| 0.42 | - |
NAD+ | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
| 0.55 | - |
dihydrolipoamide | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
| 0.63 | - |
dihydrolipoamide | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
| 0.64 | - |
dihydrolipoamide | wild-type enzyme, pH 8.0, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | E3 is a mitochondrial protein that is synthesized in precursor form in the cytoplasm. The precursor E3 for humans has a leader sequence composed of 35-amino acids at its amino-terminus, which is cleaved off during its import into the mitochondria matrix | Homo sapiens | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | Homo sapiens | - |
lipoamide + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P09622 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain XL-1 Blue by nickel affinity chromatography and dialysis | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50216, sequence calculation | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydrolipoamide dehydrogenase | - |
Homo sapiens |
| dihydrolipoamide:NAD+ oxidoreductase | - |
Homo sapiens |
| DLD | - |
Homo sapiens |
| DLDH | - |
Homo sapiens |
| E3 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 629 | - |
NAD+ | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
| 629 | - |
dihydrolipoamide | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
| 817 | - |
NAD+ | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
| 817 | - |
dihydrolipoamide | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
| 899 | - |
NAD+ | wild-type enzyme, pH 8.0, 37°C | Homo sapiens | |
| 899 | - |
dihydrolipoamide | wild-type enzyme, pH 8.0, 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | one FAD as a prosthetic group at each subunit, binding structure analysis | Homo sapiens | |
| NAD+ | binding structure analysis | Homo sapiens | |
| NADH | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | residues Pro156 and Pro303 are highly conserved | Homo sapiens |
| additional information | location of residues Pro156 and Pro303 in human E3 enzyme, structure comparisons with E3 enzymes from other species | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1143.6 | - |
dihydrolipoamide | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
| 1296.8 | - |
dihydrolipoamide | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
| 1404.7 | - |
dihydrolipoamide | wild-type enzyme, pH 8.0, 37°C | Homo sapiens | |
| 1497.6 | - |
NAD+ | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
| 3404.2 | - |
NAD+ | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
| 4731.6 | - |
NAD+ | wild-type enzyme, pH 8.0, 37°C | Homo sapiens | |
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