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Literature summary for 1.8.1.4 extracted from
- Recombinant expression, characterization and application of a dihydrolipoamide dehydrogenase with diaphorase activity from Bacillus sphaericus (2017), 3 Biotech, 7, 153 .
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | Bacillus sphaericus DLD-diaphorase exhibits considerable potential to be used as a component of diagnostic tests for the quantification of metabolites | Lysinibacillus sphaericus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DLD, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Lysinibacillus sphaericus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
recombinant His-tagged enzyme, gel filtration | Lysinibacillus sphaericus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein N6-(lipoyl)lysine + NADH + H+ | Lysinibacillus sphaericus | - |
protein N6-(dihydrolipoyl)lysine + NAD+ | - |
r |  |
| protein N6-(lipoyl)lysine + NADH + H+ | Lysinibacillus sphaericus PAD-91 | - |
protein N6-(dihydrolipoyl)lysine + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | - |
- |
- |
| Lysinibacillus sphaericus PAD-91 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | DLD catalyzes the reduction of NAD+ by dihydrolipoamide and exhibits NADH-dependent diaphorase activity | Lysinibacillus sphaericus | ? | - |
? | |
| additional information | DLD catalyzes the reduction of NAD+ by dihydrolipoamide and exhibits NADH-dependent diaphorase activity | Lysinibacillus sphaericus PAD-91 | ? | - |
? | |
| protein N6-(lipoyl)lysine + NADH + H+ | - |
Lysinibacillus sphaericus | protein N6-(dihydrolipoyl)lysine + NAD+ | - |
r | |
| protein N6-(lipoyl)lysine + NADH + H+ | - |
Lysinibacillus sphaericus PAD-91 | protein N6-(dihydrolipoyl)lysine + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, recombinant His-tagged enzyme, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydrolipoamide dehydrogenase | - |
Lysinibacillus sphaericus |
| DLD | - |
Lysinibacillus sphaericus |
| More | cf. EC 1.6.99.3 | Lysinibacillus sphaericus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | dependent on | Lysinibacillus sphaericus | |
| NADH | - |
Lysinibacillus sphaericus | |
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