Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli using the cytoplasmic expression vectors, pET3a and pET3d. Expressed as inclusion bodies and refolded by solubilisation in 8 M urea followed by dilution into a buffer containing 2 M KCl, 0.010 mM FAD, 1 mM NAD+, and 0.3 mM GSSG/3 mM GSH | Haloferax volcanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
dihydrolipoamide | 37°C, pH 8.0 | Haloferax volcanii | |
0.156 | - |
NAD+ | 37°C, pH 8.0 | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | Q04829 | - |
- |
Haloferax volcanii DSM 3757 | Q04829 | - |
- |
Purification (Comment) | Organism |
---|---|
purified using copper-based metal ion affinity chromatography in the presence of 2 M KCl | Haloferax volcanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
37°C, pH 8.0 | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Haloferax volcanii | lipoamide + NADH | - |
? | |
dihydrolipoamide + NAD+ | - |
Haloferax volcanii DSM 3757 | lipoamide + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHLipDH | - |
Haloferax volcanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Haloferax volcanii |