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Literature summary for 1.8.1.4 extracted from

  • Connaris, H.; Chaudhuri, J.B.; Danson, M.J.; Hough, D.W.
    Expression, reactivation, and purification of enzymes from Haloferax volcanii in Escherichia coli. (1999), Biotechnol. Bioeng., 64, 38-45.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli using the cytoplasmic expression vectors, pET3a and pET3d. Expressed as inclusion bodies and refolded by solubilisation in 8 M urea followed by dilution into a buffer containing 2 M KCl, 0.010 mM FAD, 1 mM NAD+, and 0.3 mM GSSG/3 mM GSH Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
dihydrolipoamide 37°C, pH 8.0 Haloferax volcanii
0.156
-
NAD+ 37°C, pH 8.0 Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii Q04829
-
-
Haloferax volcanii DSM 3757 Q04829
-
-

Purification (Commentary)

Purification (Comment) Organism
purified using copper-based metal ion affinity chromatography in the presence of 2 M KCl Haloferax volcanii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
37°C, pH 8.0 Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
Haloferax volcanii lipoamide + NADH
-
?
dihydrolipoamide + NAD+
-
Haloferax volcanii DSM 3757 lipoamide + NADH
-
?

Synonyms

Synonyms Comment Organism
DHLipDH
-
Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Haloferax volcanii