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Literature summary for 1.8.1.4 extracted from
- Histochemical staining and quantification of dihydrolipoamide dehydrogenase diaphorase activity using blue native PAGE (2007), Electrophoresis, 28, 1036-1045.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity of DLDH diaphorase is both protein amount- and time-dependent | Rattus norvegicus |
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a blue native-PAGE-based method for isolation of enzymatically active DLDH from animal tissues and visualization as well as quantification of its diaphorase activity using the NADH/nitroblue tetrazolium detection system | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Angeli's salt | at 2 mM, induces a 90% loss in DLDH diaphorase activity | Rattus norvegicus |  |
| Diethylamine NONOate | induces 71% loss in diaphorase activity at 10 mM, but does not induce any activity loss at 2 mM | Rattus norvegicus | |
| additional information | diamide, GSH, GSSG, cysteine and nitrite do not exhibit any inhibitory effects | Rattus norvegicus | |
| N-ethylmaleimide | induces more than 95% loss in DLDH diaphorase activity | Rattus norvegicus | |
| S-nitrosocysteine | induces a 62% loss in diaphorase activity at 2 mM and an 88% loss at 10 mM | Rattus norvegicus | |
| S-nitrosoglutathione | induces 84% loss in diaphorase activity at 10 mM, but does not induce any activity loss at 2 mM | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | DLDH exclusively exists in mitochondria | Rattus norvegicus | 5739 | - |
| additional information | no DLDH diaphorase activity in the cytosol | Rattus norvegicus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
adult Sprague-Dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitro blue tetrazolium + NADH | - |
Rattus norvegicus | ? + NAD+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydrolipoamide dehydrogenase | - |
Rattus norvegicus |
| DLDH | - |
Rattus norvegicus |
| DLDH dehydrogenase | - |
Rattus norvegicus |
| DLDH diaphorase | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADPH | Rattus norvegicus | |
| NADH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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