Application | Comment | Organism |
---|---|---|
degradation | conservation of the Cys-45 residue in human E3 is essential to the efficient catalytic function of the enzyme | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-Blue | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C45S | Ser-45 mutant is highly purified, shows 5270fold lower activity than wild-type enzyme. Destroyed disulfide bond between Cys-45 and Cys-50 of the active disulfide center in human E3. UV-visible spectrum of the Ser-45 mutant is similar to that of the reduced form of the enzyme and the second fluorescence emission of the mutant disappears | Homo sapiens |
C45Y | purification of the Tyr-45 mutant is not successful. Recombinant human E3 becomes too unstable to be easily obtained from Escherichia coli | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Homo sapiens |
dihydrolipoamide:NAD+ oxidoreductase | - |
Homo sapiens |
E3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains one FAD as a prosthetic group in each subunit | Homo sapiens | |
NAD+ | - |
Homo sapiens |