Literature summary for 1.8.1.4 extracted from

Rajashankar, K.R.; Bryk, R.; Kniewel, R.; Buglino, J.A.; Nathan, C.F.; Lima, C.D.
Crystal structure and functional analysis of lipoamide dehydrogenase from Mycobacterium tuberculosis (2005), J. Biol. Chem., 280, 33977-33983.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
D5A	4.3% activity of the wild type enzyme	Mycobacterium tuberculosis
E91A	49.6% activity of the wild type enzyme	Mycobacterium tuberculosis
E91K	62.5% activity of the wild type enzyme	Mycobacterium tuberculosis
F464A	5% activity of the wild type enzyme	Mycobacterium tuberculosis
H386A	20% activity of the wild type enzyme	Mycobacterium tuberculosis
H386K	9% activity of the wild type enzyme	Mycobacterium tuberculosis
H460E	69.4% activity of the wild type enzyme	Mycobacterium tuberculosis
H98A	3.3% activity of the wild type enzyme	Mycobacterium tuberculosis
K103E	17.7% activity of the wild type enzyme	Mycobacterium tuberculosis
K105A	44.7% activity of the wild type enzyme	Mycobacterium tuberculosis
K216A	58.3% activity of the wild type enzyme	Mycobacterium tuberculosis
K220A	81.2% activity of the wild type enzyme	Mycobacterium tuberculosis
K223A	67.1% activity of the wild type enzyme	Mycobacterium tuberculosis
K223E	70.5% activity of the wild type enzyme	Mycobacterium tuberculosis
K224A	55.2% activity of the wild type enzyme	Mycobacterium tuberculosis
K376A	52.2% activity of the wild type enzyme	Mycobacterium tuberculosis
K67A	67.3% activity of the wild type enzyme	Mycobacterium tuberculosis
K67E	64.3% activity of the wild type enzyme	Mycobacterium tuberculosis
K88E	74.8% activity of the wild type enzyme	Mycobacterium tuberculosis
N43A	11% activity of the wild type enzyme	Mycobacterium tuberculosis
R93A	6.5% activity of the wild type enzyme	Mycobacterium tuberculosis
R93E	3.6% activity of the wild type enzyme	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WHH9	-	-
Mycobacterium tuberculosis H37Rv	P9WHH9	-	-

Purification (Commentary)

Purification (Comment)	Organism
metal-affinity chromatography and Superdex 200 gel filtration chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dihydrolipoamide + NAD+	-	Mycobacterium tuberculosis	lipoamide + NADH + H+	-	?
dihydrolipoamide + NAD+	-	Mycobacterium tuberculosis H37Rv	lipoamide + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
LDH	-	Mycobacterium tuberculosis
lipoamide dehydrogenase	-	Mycobacterium tuberculosis
LPD	-	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Mycobacterium tuberculosis