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Literature summary for 1.8.1.4 extracted from

  • Oppermann, F.B.; Schmidt, B.; Steinbuchel, A.
    Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system (1991), J. Bacteriol., 173, 757-767.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
NADH
-
Syntrophotalea carbinolica
0.4
-
dihydrolipoamide
-
Syntrophotalea carbinolica
0.62
-
NAD+
-
Syntrophotalea carbinolica
3.7
-
Lipoamide
-
Syntrophotalea carbinolica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Syntrophotalea carbinolica
110000
-
non-denaturing gradient PAGE Syntrophotalea carbinolica

Organism

Organism UniProt Comment Textmining
Syntrophotalea carbinolica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Syntrophotalea carbinolica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.7
-
-
Syntrophotalea carbinolica

Storage Stability

Storage Stability Organism
stable for months if stored in liquid nitrogen Syntrophotalea carbinolica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH 31.1% of the activity with lipoamide Syntrophotalea carbinolica 2 ferrocyanide + NAD+ + H+
-
?
5-nitroblue tetrazolium chloride + NADH 5.1% of the activity with lipoamide Syntrophotalea carbinolica ? + NAD+
-
?
benzyl viologen + NADH 2.9% of the activity with lipoamide Syntrophotalea carbinolica ? + NAD+
-
?
dihydrolipoamide + NAD+
-
Syntrophotalea carbinolica lipoamide + NADH
-
r
menadione + NADH 13.6% of the activity with lipoamide Syntrophotalea carbinolica ? + NAD+
-
?
methylene blue + NADH 9.4% of the activity with lipoamide Syntrophotalea carbinolica ? + NAD+
-
?
oxidized 2,6-dichlorophenolindophenol + NADH 12.3% of the activity with lipoamide Syntrophotalea carbinolica ? + NAD+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Syntrophotalea carbinolica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
NADH-dependent reduction of lipoamide Syntrophotalea carbinolica

Cofactor

Cofactor Comment Organism Structure
FAD contains one noncovalently bound FAD per subunit Syntrophotalea carbinolica
NAD+ highly specific for Syntrophotalea carbinolica
NADH highly specific for Syntrophotalea carbinolica
NADP+ no activity Syntrophotalea carbinolica
NADPH no activity Syntrophotalea carbinolica