Literature summary for 1.8.1.4 extracted from

Youn, H.; Kwak, J.; Youn, H.D.; Hah, Y.C.; Kang, S.O.
Lipoamide dehydrogenase from Streptomyces seoulensis: biochemical and genetic properties (1998), Biochim. Biophys. Acta, 1388, 405-418.

Search for more literature for 1.8.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
-	Streptomyces seoulensis

Inhibitors

Inhibitors	Comment	Organism	Structure
NAD+	-	Streptomyces seoulensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.37	-	Lipoamide	-	Streptomyces seoulensis
0.4	-	1,4-benzoquinone	-	Streptomyces seoulensis
2.15	-	lipoic acid	-	Streptomyces seoulensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	2 * 54000, SDS-PAGE	Streptomyces seoulensis
110000	-	gel filtration	Streptomyces seoulensis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces seoulensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces seoulensis

Reaction

Reaction	Comment	Organism	Reaction ID
protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+	ping-pong mechanism	Streptomyces seoulensis	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.311	-	-	Streptomyces seoulensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,4-benzoquinone + NADH	-	Streptomyces seoulensis	1,4-benzoquinol + NAD+	-	?
2 ferricyanide + NADH	-	Streptomyces seoulensis	2 ferrocyanide + NAD+ + H+	-	?
2-hydroxy-1,4-benzoquinone + NADH	-	Streptomyces seoulensis	2-hydroxy-1,4-benzoquinol + NAD+	-	?
2-methyl-1,4-benzoquinone + NADH	-	Streptomyces seoulensis	2-methyl-1,4-benzoquinol + NAD+	-	?
dihydrolipoamide + NAD+	-	Streptomyces seoulensis	lipoamide + NADH	-	?
lipoic acid + NADH + H+	-	Streptomyces seoulensis	dihydrolipoic acid + NAD+	-	r
naphthoquinone + NADH	-	Streptomyces seoulensis	1,4-naphthoquinol + NAD+	-	?
oxidized 2,6-dichlorophenolindophenol + NADH	-	Streptomyces seoulensis	? + NAD+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 54000, SDS-PAGE	Streptomyces seoulensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
70	-	lipoic acid	-	Streptomyces seoulensis
88	-	Lipoamide	-	Streptomyces seoulensis
108	-	1,4-benzoquinone	-	Streptomyces seoulensis

Cofactor

Cofactor	Comment	Organism	Structure
FAD	contains 1 FAD per subunit	Streptomyces seoulensis
NAD+	-	Streptomyces seoulensis
NADH	-	Streptomyces seoulensis

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Release 2023.1 (January 2023)