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Literature summary for 1.8.1.4 extracted from
- Purification and comparative studies of dihydrolipoamide dehydrogenases from the anaerobic, glycine-utilizing bacteria Peptostreptococcus glycinophilus, Clostridium cylindrosporum, and Clostridium sporogenes (1990), J. Bacteriol., 172, 243-251.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.047 | - |
NADH | - |
Peptostreptococcus glycinophilus |  |
| 0.2 | - |
NAD+ | - |
Clostridium cylindrosporum | |
| 0.25 | - |
NAD+ | - |
Peptostreptococcus glycinophilus | |
| 0.27 | - |
NADH | - |
Clostridium cylindrosporum | |
| 0.5 | - |
NAD+ | - |
Clostridium cylindrosporum | |
| 0.83 | - |
NADP+ | - |
Clostridium cylindrosporum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Clostridium cylindrosporum |
| 53000 | - |
2 * 53000, SDS-PAGE | Peptostreptococcus glycinophilus |
| 105000 | - |
gel filtration | Clostridium cylindrosporum |
| 105000 | - |
gel filtration | Peptostreptococcus glycinophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium cylindrosporum | - |
- |
- |
| Peptostreptococcus glycinophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium cylindrosporum |
- |
Peptostreptococcus glycinophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 214 | - |
- |
Peptostreptococcus glycinophilus |
| 483 | - |
- |
Clostridium cylindrosporum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 6 months, about 10% loss of activity | Peptostreptococcus glycinophilus |
| -20°C, 6 months, about 60% loss of activity | Clostridium cylindrosporum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | - |
Clostridium cylindrosporum | lipoamide + NADH | - |
? | |
| dihydrolipoamide + NAD+ | - |
Peptostreptococcus glycinophilus | lipoamide + NADH | - |
? | |
| dihydrolipoamide + NADP+ | - |
Clostridium cylindrosporum | lipoamide + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 53000, SDS-PAGE | Peptostreptococcus glycinophilus |
| dimer | 2 * 52000, SDS-PAGE | Clostridium cylindrosporum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reaction with NAD(P)H and lipoamide | Clostridium cylindrosporum |
| 7 | - |
reaction with NAD(P)H and lipoamide | Peptostreptococcus glycinophilus |
| 9 | - |
reaction with NAD(P)+ and dihydrolipoamide | Clostridium cylindrosporum |
| 9 | - |
reaction with NAD(P)+ and dihydrolipoamide | Peptostreptococcus glycinophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 1 FAD per subunit | Clostridium cylindrosporum | |
| FAD | contains 1 FAD per subunit | Peptostreptococcus glycinophilus | |
| NAD+ | - |
Clostridium cylindrosporum | |
| NAD+ | highly specific for | Peptostreptococcus glycinophilus | |
| NADP+ | - |
Clostridium cylindrosporum | |
| NADP+ | no activity | Peptostreptococcus glycinophilus | |
| NADPH | - |
Clostridium cylindrosporum | |
| NADPH | no activity | Peptostreptococcus glycinophilus | |
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