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Literature summary for 1.8.1.14 extracted from

  • Herwald, S.; Liu, A.Y.; Zhu, B.E.; Sea, K.W.; Lopez, K.M.; Sazinsky, M.H.; Crane, E.J.
    Structure and substrate specificity of the pyrococcal coenzyme A disulfide reductases/polysulfide reductases (CoADR/Psr): implications for S(0)-based respiration and a sulfur-dependent antioxidant system in Pyrococcus (2013), Biochemistry, 52, 2764-2773.
    View publication on PubMed
Search for more literature for 1.8.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop and sitting drop vapor diffusion methods, using 100 mM Tris, pH 8.0, 2-3 M 1,6-hexanediol, and 200 mM MgCl2 Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 NADPH at 50°C and pH 8.1 in 1 M Tris buffer Pyrococcus horikoshii
0.073
-
 NADPH at 50°C and pH 8.1 in 1 M Tris buffer Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA-disulfide + NADH + H+ Pyrococcus horikoshii
-
 CoA + NAD+
-
 ?
CoA-disulfide + NADPH + H+ Pyrococcus horikoshii
-
 CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58308
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA-disulfide + NADH + H+
-
 Pyrococcus horikoshii CoA + NAD+
-
 ?
CoA-disulfide + NADPH + H+
-
 Pyrococcus horikoshii CoA + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
CoADR
-
 Pyrococcus horikoshii
coenzyme A disulfide reductase
-
 Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.2
-
 NADPH at 50°C and pH 8.1 in 1 M Tris buffer Pyrococcus horikoshii
8.1
-
 NADPH at 50°C and pH 8.1 in 1 M Tris buffer Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
coenzyme A
-
 Pyrococcus horikoshii
FAD
-
 Pyrococcus horikoshii
NADH
-
 Pyrococcus horikoshii
NADPH the enzyme shows a preference for NADPH (10fold lower Km), although it makes efficient use of NADH as well Pyrococcus horikoshii

General Information

General Information Comment Organism
physiological function the enzyme is not essential for S0 respiration in Pyrococcus but appears to participate in oxidative defense in the presence of elemental sulfur Pyrococcus horikoshii