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Literature summary for 1.8.1.13 extracted from
- The novel disulfide reductase bis-gamma-glutamylcystine reductase and dihydrolipoamide dehydrogenase from Halobacterium halobium: Purification by immobilized-metal-ion affinity chromatography and properties of the enzymes (1988), J. Bacteriol., 170, 3459-3467.
General Stability
| General Stability | Organism |
|---|---|
| high-ionic-strength buffer above 2 M stabilizes during purification and storage | Halobacterium salinarum |
| high-ionic-strength buffer above 3.5 M increases thermal stability | Halobacterium salinarum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
2 * 61000, SDS-PAGE | Halobacterium salinarum |
| 122000 | - |
gel filtration | Halobacterium salinarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bis-gamma-glutamylcystine + NADPH + H+ | Halobacterium salinarum | - |
2 gamma-glutamylcysteine + NADP+ | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium salinarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Halobacterium salinarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Halobacterium salinarum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, stable to prolonged storage at room temperature in high-ionic-strength buffer above 3.5 M | Halobacterium salinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bis-gamma-glutamylcystine + NADPH + H+ | - |
Halobacterium salinarum | 2 gamma-glutamylcysteine + NADP+ | - |
? | |
| additional information | no activity detected with GSSG | Halobacterium salinarum | ? | - |
? | |
| additional information | lipoic acid disulfide and dihydrolipoamide are no substrates | Halobacterium salinarum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 61000, SDS-PAGE | Halobacterium salinarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GCR | - |
Halobacterium salinarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
retains full activity after incubation in high-and intermediate-ionic-strength buffers, inactivated after incubation in low-ionic strength buffers | Halobacterium salinarum |
| 80 | - |
inactivation in buffers of each of the 3 ionic strengths, low-ionic, intermediate-ionic and high-ionic | Halobacterium salinarum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Halobacterium salinarum | |
| NADH | less effective than NADPH | Halobacterium salinarum | |
| NADPH | - |
Halobacterium salinarum | |
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