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Literature summary for 1.8.1.12 extracted from

  • Toro, M.A.; Sanchez-Murcia, P.A.; Moreno, D.; Ruiz-Santaquiteria, M.; Alzate, J.F.; Negri, A.; Camarasa, M.J.; Gago, F.; Velazquez, S.; Jimenez-Ruiz, A.
    Probing the dimerization interface of Leishmania infantum trypanothione reductase with site-directed mutagenesis and short peptides (2013), ChemBioChem, 14, 1212-1217.
    View publication on PubMed
Search for more literature for 1.8.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leishmania infantum

Protein Variants

Protein Variants Comment Organism
E436A the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Leishmania infantum
Q439A the mutant shows slightly increased catalytic efficiency compared to the wild type enzyme Leishmania infantum
W81A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Leishmania infantum

Inhibitors

Inhibitors Comment Organism Structure
Ac-PAIIQSVGISNLKNL-NH2 about 30% residual activity at 0.025 mM Leishmania infantum
Ac-PEIIQSVGISMKM-NH2
-
 Leishmania infantum
Ac-PEIIQSVGISNLKNL-NH2
-
 Leishmania infantum
Ac-PKAIQSVGISNLKNL-NH2 about 18% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIASVGISNLKNL-NH2 about 65% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQAVGISNLKNL-NH2 about 10% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQSAGISNLKNL-NH2 about 19% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQSVAISNLKNL-NH2 about 20% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQSVGASNLKNL-NH2 about 22% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQSVGIANLKNL-NH2 about 15% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQSVGISAKNL-NH2 about 30% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQSVGISMKM-NH2 the enzyme is almost completely inactivated after 50 min (3000 s) in the presence of 0.025 mM Leishmania infantum
Ac-PKIIQSVGISNLANL-NH2 about 31% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQSVGISNLKA-NH2 about 31% residual activity at 0.025 mM Leishmania infantum
Ac-PKIIQSVGISNLKNL-NH2 about 20% residual activity at 0.025 mM Leishmania infantum
additional information not inhibited by Ac-KMGISSMVQPKII-NH2 and Ac-KIIPKVQGMSIMS-NH2 Leishmania infantum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0206
-
 trypanothione disulfide wild type enzyme, pH and temperature not specified in the publication Leishmania infantum
0.0253
-
 trypanothione disulfide mutant enzyme Q439A, pH and temperature not specified in the publication Leishmania infantum
0.0493
-
 trypanothione disulfide mutant enzyme W81A, pH and temperature not specified in the publication Leishmania infantum
0.1922
-
 trypanothione disulfide mutant enzyme E436A, pH and temperature not specified in the publication Leishmania infantum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Leishmania infantum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trypanothione disulfide + NADPH + H+ Leishmania infantum
-
 trypanothione + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania infantum A4HSF7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leishmania infantum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trypanothione disulfide + NADPH + H+
-
 Leishmania infantum trypanothione + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Leishmania infantum

Synonyms

Synonyms Comment Organism
trypanothione reductase
-
 Leishmania infantum
TryR
-
 Leishmania infantum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 trypanothione disulfide mutant enzyme E436A, pH and temperature not specified in the publication Leishmania infantum
16.6
-
 trypanothione disulfide mutant enzyme W81A, pH and temperature not specified in the publication Leishmania infantum
30.2
-
 trypanothione disulfide wild type enzyme, pH and temperature not specified in the publication Leishmania infantum
40.8
-
 trypanothione disulfide mutant enzyme Q439A, pH and temperature not specified in the publication Leishmania infantum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Leishmania infantum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0157
-
 pH and temperature not specified in the publication Leishmania infantum Ac-PKIIQSVGISNLKNL-NH2
0.0179
-
 pH and temperature not specified in the publication Leishmania infantum Ac-PKIIQSVGISMKM-NH2
0.0336
-
 pH and temperature not specified in the publication Leishmania infantum Ac-PEIIQSVGISNLKNL-NH2
0.0408
-
 pH and temperature not specified in the publication Leishmania infantum Ac-PEIIQSVGISMKM-NH2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
 trypanothione disulfide mutant enzyme E436A, pH and temperature not specified in the publication Leishmania infantum
300
-
 trypanothione disulfide mutant enzyme W81A, pH and temperature not specified in the publication Leishmania infantum
1500
-
 trypanothione disulfide wild type enzyme, pH and temperature not specified in the publication Leishmania infantum
1600
-
 trypanothione disulfide mutant enzyme Q439A, pH and temperature not specified in the publication Leishmania infantum