Application | Comment | Organism |
---|---|---|
pharmacology | enzyme is a target for selective drug design | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, reduced by NADPH, inactivated by binding of quinacrine mustard, 13 mg/ml enzyme-inhibitor complex in Na+ malate, pH 6.0, with precipitant 20% w/v PEG 8000, X-ray diffraction structure determination and analysis at 2.7 A resolution | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MelT | adduct of melarsen oxide with dihydrotrypanothione | Trypanosoma cruzi | |
Quinacrine | competitive | Trypanosoma cruzi | |
quinacrine mustard | enzyme and inhibitor first forms a reversible complex, then irreversible inactivation of NADPH-reduced and oxidized enzyme occurs, enzyme contains 2 interacting binding sites in the active site for the inhibitors, 2 inhibitor molecules per enzyme monomer are bound, addition of 2-mercaptoethanol prevents inactivation up to 25 mM, clomipramine strongly protects th enzyme from inactivation | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH | Trypanosoma cruzi | kex enzyme in the tryanothione-based redox metabolism of pathogenic trypanosomes | trypanothione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Homo sapiens | - |
- |
- |
Trypanosoma cruzi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
? | |
trypanothione disulfide + NADPH | kex enzyme in the tryanothione-based redox metabolism of pathogenic trypanosomes | Trypanosoma cruzi | trypanothione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
trypanothione reductase | - |
Trypanosoma cruzi |
TryR | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is a flavoprotein | Trypanosoma cruzi | |
NADPH | - |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Trypanosoma cruzi | |
0.012 | - |
MelT | pH 7.5, 27°C | Trypanosoma cruzi |