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Literature summary for 1.8.1.12 extracted from
- Phenotype of recombinant Leishmania donovani and Trypanosoma cruzi which over-express trypanothione reductase. Sensitivity towards agents that are thought to induce oxidative stress (1993), Eur. J. Biochem., 218, 29-37.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is dependent on a redox-active cysteine at the active centre | Leishmania donovani | |
| additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into expression vector and overexpressed in Leishmania donovani and in Trypanosoma cruzi | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Gentian violet | wild-type and recombinant enzyme overexpressing cells | Leishmania donovani |  |
| H2O2 | wild-type and recombinant enzyme overexpressing cells | Leishmania donovani | |
| nifurtimox | wild-type and recombinant enzyme overexpressing cells | Leishmania donovani | |
| nifurtimox | - |
Trypanosoma cruzi | |
| nitrofurazone | wild-type and recombinant enzyme overexpressing cells | Leishmania donovani | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trypanothione disulfide + NADPH | Trypanosoma cruzi | - |
trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | Leishmania donovani | scheme for trypanothione-dependent H2O2 metabolism | trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | Leishmania donovani | metabolizes H2O2, recombinant and wild-type cells | trypanothione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | - |
- |
- |
| Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant overexpression from plasmid in Leishmania donovani and in Trypanosoma cruzi | Leishmania donovani |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.06 | - |
- |
Leishmania donovani |
| 0.22 | - |
- |
Trypanosoma cruzi |
| 0.55 | - |
crude extract cells overexpressing the recombinant enzyme | Leishmania donovani |
| 2.15 | - |
recombinant overexpressed in Trypanosoma cruzi cells | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trypanothione disulfide + NADPH | - |
Leishmania donovani | trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | scheme for trypanothione-dependent H2O2 metabolism | Leishmania donovani | trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | metabolizes H2O2, recombinant and wild-type cells | Leishmania donovani | trypanothione + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TR | - |
Leishmania donovani |
| TR | - |
Trypanosoma cruzi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Leishmania donovani | |
| FAD | - |
Trypanosoma cruzi | |
| NADPH | - |
Leishmania donovani | |
| NADPH | - |
Trypanosoma cruzi | |
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Release 2023.1 (January 2023)
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