Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is dependent on a redox-active cysteine at the active centre | Crithidia fasciculata | |
additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi | |
additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma congolense |
Application | Comment | Organism |
---|---|---|
medicine | - |
Crithidia fasciculata |
medicine | good target for structure/function studies and trypanicidal inhibitor design | Trypanosoma cruzi |
medicine | good target for structure/function studies and trypanicidal inhibitor design | Trypanosoma congolense |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, sequence analysis | Trypanosoma congolense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | 0.007 | NADPH | - |
Crithidia fasciculata | |
0.005 | 0.007 | NADPH | - |
Trypanosoma cruzi | |
0.005 | 0.007 | NADPH | - |
Trypanosoma congolense | |
0.031 | - |
trypanothione disulfide | - |
Trypanosoma congolense | |
0.045 | - |
trypanothione disulfide | - |
Trypanosoma cruzi | |
0.051 | - |
trypanothione disulfide | - |
Crithidia fasciculata | |
0.096 | - |
NADH | - |
Trypanosoma congolense | |
0.12 | - |
deazatrypanothione disulfide | - |
Trypanosoma congolense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Trypanosoma cruzi |
54000 | - |
2 * 54000, SDS-PAGE | Crithidia fasciculata |
54000 | - |
2 * 54000, SDS-PAGE | Trypanosoma congolense |
110000 | - |
- |
Crithidia fasciculata |
110000 | - |
recombinant enzyme, gel filtration | Trypanosoma congolense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
insect-parasitic trypanosomatid | - |
Trypanosoma congolense | - |
- |
- |
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Trypanosoma congolense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.9 | - |
purified enzyme | Trypanosoma congolense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deazatrypanothione + NADPH | substrate analogue | Trypanosoma congolense | ? | - |
? | |
deazatrypanothione disulfide + NADPH + H+ | - |
Trypanosoma congolense | ? | - |
? | |
deazatrypanothione disulfide + NADPH + H+ | - |
Trypanosoma congolense | ? | - |
r | |
trypanothione disulfide + NADPH | - |
Crithidia fasciculata | trypanothione + NADP+ | - |
r | |
trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
r | |
trypanothione disulfide + NADPH | - |
Trypanosoma congolense | trypanothione + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Trypanosoma cruzi |
dimer | 2 * 54000, SDS-PAGE | Crithidia fasciculata |
dimer | 2 * 54000, SDS-PAGE | Trypanosoma congolense |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83.3 | - |
deazatrypanothione disulfide | - |
Trypanosoma congolense | |
160 | - |
trypanothione disulfide | - |
Trypanosoma congolense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Crithidia fasciculata | |
FAD | - |
Trypanosoma cruzi | |
FAD | - |
Trypanosoma congolense | |
NADH | - |
Trypanosoma cruzi | |
NADH | 7% activity of that with NADPH | Trypanosoma congolense | |
NADP+ | - |
Crithidia fasciculata | |
NADP+ | - |
Trypanosoma cruzi | |
NADP+ | - |
Trypanosoma congolense | |
NADPH | - |
Crithidia fasciculata | |
NADPH | enzyme also utilizes NADH, but less efficiently | Trypanosoma cruzi | |
NADPH | enzyme also utilizes NADH, but less efficiently | Trypanosoma congolense |