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Literature summary for 1.8.1.12 extracted from

  • Sullivan, F.X.; Shames, S.L.; Walsh, C.T.
    Expression of Trypanosoma congolense trypanothione reductase in Escherichia coli: overproduction, purification, and characterization (1989), Biochemistry, 28, 4986-4992.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information activity is dependent on a redox-active cysteine at the active centre Crithidia fasciculata
additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma cruzi
additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma congolense

Application

Application Comment Organism
medicine
-
Crithidia fasciculata
medicine good target for structure/function studies and trypanicidal inhibitor design Trypanosoma cruzi
medicine good target for structure/function studies and trypanicidal inhibitor design Trypanosoma congolense

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, sequence analysis Trypanosoma congolense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005 0.007 NADPH
-
Crithidia fasciculata
0.005 0.007 NADPH
-
Trypanosoma cruzi
0.005 0.007 NADPH
-
Trypanosoma congolense
0.031
-
trypanothione disulfide
-
Trypanosoma congolense
0.045
-
trypanothione disulfide
-
Trypanosoma cruzi
0.051
-
trypanothione disulfide
-
Crithidia fasciculata
0.096
-
NADH
-
Trypanosoma congolense
0.12
-
deazatrypanothione disulfide
-
Trypanosoma congolense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Trypanosoma cruzi
54000
-
2 * 54000, SDS-PAGE Crithidia fasciculata
54000
-
2 * 54000, SDS-PAGE Trypanosoma congolense
110000
-
-
Crithidia fasciculata
110000
-
recombinant enzyme, gel filtration Trypanosoma congolense

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata
-
insect-parasitic trypanosomatid
-
Trypanosoma congolense
-
-
-
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Trypanosoma congolense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.9
-
purified enzyme Trypanosoma congolense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deazatrypanothione + NADPH substrate analogue Trypanosoma congolense ?
-
?
deazatrypanothione disulfide + NADPH + H+
-
Trypanosoma congolense ?
-
?
deazatrypanothione disulfide + NADPH + H+
-
Trypanosoma congolense ?
-
r
trypanothione disulfide + NADPH
-
Crithidia fasciculata trypanothione + NADP+
-
r
trypanothione disulfide + NADPH
-
Trypanosoma cruzi trypanothione + NADP+
-
r
trypanothione disulfide + NADPH
-
Trypanosoma congolense trypanothione + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Trypanosoma cruzi
dimer 2 * 54000, SDS-PAGE Crithidia fasciculata
dimer 2 * 54000, SDS-PAGE Trypanosoma congolense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83.3
-
deazatrypanothione disulfide
-
Trypanosoma congolense
160
-
trypanothione disulfide
-
Trypanosoma congolense

Cofactor

Cofactor Comment Organism Structure
FAD
-
Crithidia fasciculata
FAD
-
Trypanosoma cruzi
FAD
-
Trypanosoma congolense
NADH
-
Trypanosoma cruzi
NADH 7% activity of that with NADPH Trypanosoma congolense
NADP+
-
Crithidia fasciculata
NADP+
-
Trypanosoma cruzi
NADP+
-
Trypanosoma congolense
NADPH
-
Crithidia fasciculata
NADPH enzyme also utilizes NADH, but less efficiently Trypanosoma cruzi
NADPH enzyme also utilizes NADH, but less efficiently Trypanosoma congolense