BRENDA - Enzyme Database show
show all sequences of 1.8.1.12

Mechanism of inhibition of trypanothione reductase and glutathione reductase by trivalent organic arsenicals

Cunningham, M.L.; Zvelebil, M.J.J.M.; Fairlamb, A.H.; Eur. J. Biochem. 221, 285-295 (1994)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
activity is dependent on a redox-active cysteine at the active centre
Crithidia fasciculata
General Stability
General Stability
Organism
loss of 39% activity after incubation with NADPH at 25°C
Crithidia fasciculata
Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
+ NADPH, slightly
Crithidia fasciculata
cymelarsen
+ NADPH
Crithidia fasciculata
Melarsen oxide
inhibition is increased in presence of NADPH, inhibition is abolished by melarsen-trypanothione disulfide adduct
Crithidia fasciculata
melarsen-trypanothione disulfide adduct
no inhibition
Crithidia fasciculata
melarsoprol
+ NADPH, slightly
Crithidia fasciculata
NADPH
NADPH inhibits the enzyme at higher concentration and during longer incubation times, enzyme can be protected by NADP+
Crithidia fasciculata
phenylarsenoxide
+ NADPH
Crithidia fasciculata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Crithidia fasciculata
-
-
-
Purification (Commentary)
Commentary
Organism
-
Crithidia fasciculata
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
247
-
purified enzyme
Crithidia fasciculata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trypanothione disulfide + NADPH
-
394761
Crithidia fasciculata
trypanothione + NADP+
-
394761
Crithidia fasciculata
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Crithidia fasciculata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Crithidia fasciculata
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Crithidia fasciculata
NADPH
-
Crithidia fasciculata
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.016
-
Melarsen oxide
with melarsen-trypanothione disulfide-adduct
Crithidia fasciculata
0.0172
-
Melarsen oxide
with NADPH
Crithidia fasciculata
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
activity is dependent on a redox-active cysteine at the active centre
Crithidia fasciculata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Crithidia fasciculata
NADPH
-
Crithidia fasciculata
General Stability (protein specific)
General Stability
Organism
loss of 39% activity after incubation with NADPH at 25°C
Crithidia fasciculata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
+ NADPH, slightly
Crithidia fasciculata
cymelarsen
+ NADPH
Crithidia fasciculata
Melarsen oxide
inhibition is increased in presence of NADPH, inhibition is abolished by melarsen-trypanothione disulfide adduct
Crithidia fasciculata
melarsen-trypanothione disulfide adduct
no inhibition
Crithidia fasciculata
melarsoprol
+ NADPH, slightly
Crithidia fasciculata
NADPH
NADPH inhibits the enzyme at higher concentration and during longer incubation times, enzyme can be protected by NADP+
Crithidia fasciculata
phenylarsenoxide
+ NADPH
Crithidia fasciculata
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.016
-
Melarsen oxide
with melarsen-trypanothione disulfide-adduct
Crithidia fasciculata
0.0172
-
Melarsen oxide
with NADPH
Crithidia fasciculata
Purification (Commentary) (protein specific)
Commentary
Organism
-
Crithidia fasciculata
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
247
-
purified enzyme
Crithidia fasciculata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trypanothione disulfide + NADPH
-
394761
Crithidia fasciculata
trypanothione + NADP+
-
394761
Crithidia fasciculata
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Crithidia fasciculata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Crithidia fasciculata
Other publictions for EC 1.8.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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4
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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-
1
1
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-
-
1
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-
1
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-
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743345
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Trypanosoma cruzi
Nat. Prod. Commun.
11
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2016
-
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1
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-
6
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3
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1
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6
-
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742920
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J. Biomol. Struct. Dyn.
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1
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1
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3
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1
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1
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-
-
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-
-
-
-
-
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-
-
-
-
743571
Beig
Trypanothione reductase a tar ...
Trypanosoma brucei brucei
PLoS Negl. Trop. Dis.
9
e0003773
2015
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-
-
-
-
3
-
-
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-
2
-
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3
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3
3
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-
-
-
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-
-
-
-
-
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-
724758
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1
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3
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16
4
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1
1
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
4
-
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-
1
-
-
4
-
-
1
1
-
3
-
4
16
-
4
-
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1
1
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1
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-
1
1
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-
-
4
-
-
-
-
-
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4
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1
1
-
-
1
3
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1
-
3
-
-
1
-
-
1
-
-
1
-
-
-
-
3
-
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-
2
1
-
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-
1
2
1
-
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-
1
1
3
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
724772
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Leishmania infantum
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-
-
-
-
-
-
12
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
12
-
12
-
-
-
2
-
-
-
12
12
12
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725064
Lazarin-Bidoia
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60
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2013
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-
-
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1
-
-
-
-
1
-
4
-
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-
3
-
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1
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1
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1
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1
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1
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3
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
725848
Mutlu
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J. Vector Borne Dis.
50
38-44
2013
-
-
-
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1
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3
-
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1
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2
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1
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2
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1
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-
1
-
-
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-
-
-
-
1
-
1
1
-
-
-
742609
Ferreira
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Future Med. Chem.
5
1753-1762
2013
-
-
-
1
-
-
-
-
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-
-
-
3
-
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-
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1
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-
-
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-
-
-
-
-
-
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-
-
-
724552
Rodrigues
Investigation of trypanothione ...
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Bioorg. Med. Chem.
20
1760-1766
2012
-
-
-
-
-
-
4
-
-
-
-
8
-
19
-
-
-
-
-
8
-
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
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4
-
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-
8
-
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-
-
8
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711756
Walton
Synthesis and evaluation of in ...
Trypanosoma brucei
ChemMedChem
6
321-328
2011
-
-
-
-
-
-
20
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
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-
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-
1
-
-
18
-
-
-
1
-
-
-
18
20
-
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-
-
1
-
-
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-
-
-
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-
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-
-
-
711757
Eberle
Improved inhibitors of trypano ...
Leishmania donovani, Plasmodium falciparum, Trypanosoma brucei rhodesiense, Trypanosoma cruzi
ChemMedChem
6
292-301
2011
-
-
-
-
-
-
56
-
-
-
-
-
-
7
-
-
-
-
-
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-
4
-
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-
8
3
-
42
-
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8
-
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-
42
56
3
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4
-
-
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-
723967
Shukla
Biophysical and folding parame ...
Leishmania infantum
Appl. Biochem. Biotechnol.
165
13-23
2011
-
-
1
-
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1
-
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1
-
4
-
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1
-
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1
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1
1
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2
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1
2
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1
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1
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1
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1
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1
1
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-
700087
Jones
Comparative structural, kineti ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
169
12-19
2010
-
-
1
1
-
-
10
2
-
-
4
-
-
4
-
-
1
-
-
-
-
-
3
1
-
-
-
1
-
-
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2
-
-
6
-
-
1
2
1
-
-
6
10
-
2
-
-
4
-
-
-
-
1
-
-
-
-
3
1
-
-
-
1
-
-
-
-
-
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-
-
1
1
712584
Venkatesan
Molecular docking studies of s ...
Leishmania infantum
J. Comput. Chem.
31
2463-2475
2010
-
-
-
-
-
-
45
-
-
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-
-
3
-
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-
1
-
-
-
-
-
-
-
-
2
4
-
-
-
-
-
2
-
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-
-
45
4
-
-
-
-
-
-
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696460
Rai
Leishmania donovani trypanothi ...
Leishmania donovani
Biochim. Biophys. Acta
1794
1474-1484
2009
-
-
1
-
-
1
1
-
-
-
-
-
-
4
-
-
1
-
1
2
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
1
-
1
-
-
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-
-
-
-
-
-
1
1
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696679
Cavalli
Privileged structure-guided sy ...
Trypanosoma cruzi
Bioorg. Med. Chem. Lett.
19
3031-3035
2009
-
-
-
-
-
-
8
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
1
4
-
-
-
-
-
1
-
-
-
-
8
4
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697321
Stump
Pentafluorosulfanyl as a novel ...
Trypanosoma cruzi
ChemBioChem
10
79-83
2009
-
-
-
-
-
-
3
-
-
-
-
-
-
1
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-
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1
4
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1
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3
4
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-
-
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-
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-
-
697338
Richardson
Improved tricyclic inhibitors ...
Trypanosoma brucei
ChemMedChem
4
1333-1340
2009
-
-
-
-
-
-
23
-
-
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4
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2
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23
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2
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23
23
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-
-
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-
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-
-
-
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-
-
697339
Patterson
Synthesis and evaluation of 1- ...
Trypanosoma brucei, Trypanosoma brucei BSF 427
ChemMedChem
4
1341-1353
2009
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-
-
-
-
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32
-
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-
4
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4
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1
1
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31
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1
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31
32
1
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4
-
-
-
-
-
-
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-
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-
-
-
-
-
-
697340
Eberle
Synthesis, inhibition potency, ...
Plasmodium falciparum, Trypanosoma brucei rhodesiense, Trypanosoma brucei rhodesiense STIB900, Trypanosoma cruzi, Trypanosoma cruzi Tulahuen C4
ChemMedChem
4
2034-2044
2009
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-
93
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-
-
11
-
-
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6
-
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4
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-
6
-
-
51
-
-
-
6
-
-
-
51
93
-
-
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-
-
-
-
-
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699454
Perez-Pineiro
Development of a novel virtual ...
Trypanosoma brucei, Trypanosoma cruzi
J. Med. Chem.
52
1670-1680
2009
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-
-
-
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38
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4
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-
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2
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4
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34
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4
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34
38
-
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-
-
-
-
2
-
-
-
-
-
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-
-
-
-
-
-
699618
da Rocha Pita
Molecular docking of a series ...
Trypanosoma cruzi
J. Mol. Graph. Model.
28
330-335
2009
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-
-
-
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-
21
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3
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1
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2
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2
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21
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699619
Iribarne
Assaying phenothiazine derivat ...
Trypanosoma cruzi
J. Mol. Graph. Model.
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371-381
2009
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-
-
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51
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3
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2
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8
-
-
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51
8
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-
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701090
Baiocco
Trypanothione reductase from L ...
Leishmania infantum
Protein Pept. Lett.
16
196-200
2009
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-
1
1
-
-
-
-
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5
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1
-
-
-
-
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1
-
1
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1
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-
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-
-
-
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-
-
-
-
-
-
-
-
710883
Holloway
Trypanothione reductase high-t ...
Trypanosoma cruzi, Trypanosoma cruzi Silvio X10/1
Antimicrob. Agents Chemother.
53
2824-2833
2009
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-
-
-
-
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25
1
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-
-
-
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2
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2
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2
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20
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2
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20
25
-
1
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711751
Spinks
Investigation of trypanothione ...
Trypanosoma brucei
ChemMedChem
4
2060-2069
2009
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39
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3
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1
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2
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37
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2
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-
37
39
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-
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-
1
-
-
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-
-
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684759
Castro-Pinto
Cloning and expression of tryp ...
Leishmania amazonensis
Arch. Microbiol.
189
375-384
2008
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1
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-
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2
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8
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1
-
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2
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-
1
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-
1
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-
2
-
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-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
684776
Blackie
-
Inhibition of trypanothione re ...
Leishmania donovani, Trypanosoma brucei rhodesiense, Trypanosoma cruzi
ARKIVOC
2008
52-60
2008
-
3
-
-
-
-
25
-
-
-
-
-
-
3
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-
-
-
-
-
-
-
3
-
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12
-
3
-
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-
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-
12
25
-
-
-
-
-
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-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
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685553
Galarreta
The use of natural product sca ...
Leishmania amazonensis, Trypanosoma cruzi
Bioorg. Med. Chem.
16
6689-6695
2008
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-
-
-
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-
7
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4
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-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
1
7
3
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685798
Singh
Modeled structure of trypanoth ...
Leishmania infantum
BMB Rep.
41
444-447
2008
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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-
1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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686809
Cota
Altenusin, a biphenyl isolated ...
Leishmania amazonensis, Trypanosoma cruzi
FEMS Microbiol. Lett.
285
177-182
2008
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-
-
-
-
-
4
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-
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9
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-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
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-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688813
Cota
A potent trypanocidal componen ...
Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
103
263-270
2008
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1
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
3
5
-
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-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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696729
Porcal
New trypanocidal hybrid compou ...
Trypanosoma cruzi
Bioorg. Med. Chem.
16
6995-7004
2008
-
-
-
-
-
-
3
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
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700542
Stump
Diaryl sulfide-based inhibitor ...
Plasmodium falciparum, Trypanosoma brucei rhodesiense, Trypanosoma brucei rhodesiense STIB900
Org. Biomol. Chem.
6
3935-3947
2008
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-
-
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58
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3
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7
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-
-
-
-
-
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3
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-
-
-
-
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4
3
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17
-
-
-
4
-
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17
58
3
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
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673491
Ondarza
The effects by neuroleptics, a ...
Naegleria fowleri
Exp. Parasitol.
115
41-47
2007
4
-
-
-
-
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5
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4
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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685567
Martyn
High-throughput screening affo ...
Trypanosoma brucei
Bioorg. Med. Chem. Lett.
17
1280-1283
2007
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1
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-
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13
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3
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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13
-
1
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-
-
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-
13
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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686095
Stump
Betraying the parasites redox ...
Trypanosoma brucei rhodesiense
ChemMedChem
2
1708-1712
2007
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1
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-
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-
10
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1
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2
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-
-
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8
-
4
-
1
-
-
-
-
-
4
10
8
-
-
-
-
-
-
-
-
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-
-
-
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2
-
-
-
-
-
-
-
-
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688034
Castro-Pinto
Leishmania amazonensis trypano ...
Leishmania amazonensis
J. Enzyme Inhib. Med. Chem.
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71-75
2007
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1
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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688574
Czechowicz
The synthesis and inhibitory a ...
Trypanosoma cruzi
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3689-3693
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-
-
-
-
1
-
-
-
-
-
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-
-
-
3
-
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-
1
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690113
Iribarne
-
Interaction energies of nitrof ...
Trypanosoma cruzi
Theochem
818
7-22
2007
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1
-
-
-
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7
-
-
-
-
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1
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-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
7
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
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672537
Hamilton
Time-dependent inhibitors of t ...
Lunaria biennis
Bioorg. Med. Chem.
14
2266-2278
2006
-
-
-
-
-
-
12
-
-
-
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3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
10
-
3
-
-
-
1
-
-
-
3
12
10
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672614
Holloway
Discovery of 2-iminobenzimidaz ...
Trypanosoma brucei, Trypanosoma brucei rhodesiense
Bioorg. Med. Chem. Lett.
17
1422-1427
2006
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1
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34
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4
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-
-
-
-
-
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2
-
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-
-
-
-
-
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1
3
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34
-
1
-
1
-
-
-
34
34
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
-
-
-
-
672855
Romao
Glutathione and the redox cont ...
Leishmania amazonensis, Leishmania amazonensis MPRO/BR/72/M1841-LV-79, Leishmania braziliensis, Leishmania braziliensis MHOM/BR/75/M2904, Leishmania donovani, Leishmania donovani MHOM/ET/67/HU3, Leishmania major, Leishmania major LV-39
Braz. J. Med. Biol. Res.
39
355-363
2006
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14
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-
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-
-
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8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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8
-
-
-
-
-
-
-
-
-
-
-
-
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673488
Ondarza
Naegleria fowleri: a free-livi ...
Naegleria fowleri
Exp. Parasitol.
114
141-146
2006
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675780
de Oliveira
Arylfurans as potential Trypan ...
Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
101
169-173
2006
-
-
-
-
-
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12
-
-
-
-
-
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2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
2
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658318
Tamayo
Trypanothione reductase from t ...
Entamoeba histolytica
Biotechnol. Appl. Biochem.
41
105-115
2005
-
1
1
-
-
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1
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5
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1
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2
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1
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1
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1
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2
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1
1
2
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1
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2
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1
-
1
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-
1
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-
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-
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660445
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Expression, purification, and ...
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1
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4
3
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2
4
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6
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1
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-
1
-
6
2
1
-
-
1
1
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-
2
-
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1
1
2
-
-
-
-
4
-
3
-
-
2
4
-
-
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1
-
-
1
-
6
2
1
-
-
1
1
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-
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672529
Dixon
Mechanism and structure-activi ...
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1
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-
-
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1
21
-
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-
1
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21
21
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-
1
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-
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-
1
-
-
-
-
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1
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1
2
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1
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6
2
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-
1
-
-
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675289
Lee
Irreversible inactivation of t ...
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1
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1
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1
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6
-
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1
-
-
-
-
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Antitrypanosomal, antileishman ...
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1
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1
18
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6
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1
-
-
-
6
21
18
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-
-
-
-
-
-
-
-
-
-
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1
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-
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Two interacting binding sites ...
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1
1
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1
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-
2
2
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1
-
2
1
-
-
-
3
2
-
-
-
-
1
-
-
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-
-
-
-
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2
1
1
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1
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Trypanothione reductase activi ...
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2
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1
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-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
7
-
-
2
-
-
-
-
-
1
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-
-
-
-
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658262
Hamilton
Benzofuranyl 3,5-bis-polyamine ...
Trypanosoma cruzi
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2
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-
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2
1
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-
2
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5
1
-
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-
2
-
-
-
-
-
-
-
-
2
-
-
-
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659676
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Coupling of a competitive and ...
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-
-
-
-
-
3
-
1
-
-
-
1
-
-
2
1
-
-
-
-
-
2
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
394803
Salmon-Chemin
2- And 3-substituted 1,4-napht ...
Leishmania donovani, Leishmania infantum, Trypanosoma brucei, Trypanosoma cruzi
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3
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18
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1
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-
-
-
10
-
-
-
-
-
-
-
-
8
-
-
-
3
3
-
8
-
-
-
-
18
-
-
-
-
-
4
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
394790
Garrard
Inhibition of trypanothione re ...
Trypanosoma cruzi
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3639-3642
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5
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-
1
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-
-
-
-
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2
4
-
-
1
-
-
2
-
-
-
-
5
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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394804
Bonse
(2,2':6',2''-Terpyridine)plati ...
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1
-
-
-
-
-
-
-
-
2
-
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-
1
-
-
2
-
-
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
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394778
Montrichard
Evidence for the co-existence ...
Euglena gracilis, Euglena gracilis Z
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2
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1
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1
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-
3
2
-
4
1
-
-
-
2
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
3
2
-
4
1
-
-
-
2
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394779
Gallwitz
Ajoene is an inhibitor and sub ...
Trypanosoma cruzi
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1
-
-
-
-
2
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
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394791
Blumenstiel
Nitrofuran drugs as common sub ...
Trypanosoma congolense, Trypanosoma cruzi
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58
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1
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-
-
-
-
-
6
-
1
-
-
-
-
-
-
4
1
-
-
2
2
-
4
-
-
-
-
8
1
1
-
-
-
2
-
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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394793
Bond
Crystal structure of Trypanoso ...
Trypanosoma cruzi
Structure
7
81-89
1999
1
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1
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1
-
2
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-
-
-
4
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-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
1
1
-
2
1
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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394794
Davioud-Charvet
Synthesis of 5,5'-dithiobis(2- ...
Trypanosoma cruzi
Anal. Biochem.
268
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1999
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3
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1
-
2
-
1
-
-
3
1
-
-
2
1
-
-
1
1
1
2
-
-
-
-
2
1
3
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
3
1
-
-
-
-
-
-
-
-
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394805
Bonse
Inhibition of Trypanosoma cruz ...
Trypanosoma cruzi
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1
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-
-
-
1
-
1
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-
-
-
-
-
2
5
-
-
1
1
-
2
-
-
-
-
5
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
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394807
Tovar
Evidence that trypanothione re ...
Leishmania donovani
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653-660
1998
1
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1
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2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
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394808
Fournet
Trypanocidal bisbenzylisoquino ...
Trypanosoma cruzi
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-
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-
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-
1
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
2
-
-
-
-
3
2
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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394776
Stoll
Glutathione reductase turned i ...
Homo sapiens, Trypanosoma cruzi
Biochemistry
36
6437-6447
1997
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2
1
1
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2
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5
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1
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-
-
2
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-
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-
1
2
-
-
4
-
-
-
-
-
2
4
1
1
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
1
2
-
-
-
-
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394809
Bonnet
New spermine and spermidine de ...
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5
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1997
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4
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-
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-
1
-
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-
1
-
-
2
2
-
-
-
-
-
2
-
-
-
-
4
2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
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-
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394792
Zheng
Catalytic and potentiometric c ...
Trypanosoma congolense
Biochemistry
34
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1
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2
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6
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1
1
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4
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9
-
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-
4
8
-
-
1
-
1
4
-
2
-
-
4
8
6
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-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
9
-
-
-
-
-
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394798
Ponasik
-
Kukoamine A and other hydropho ...
Crithidia fasciculata
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1995
1
1
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3
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2
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-
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-
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2
3
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1
1
-
2
-
-
-
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3
3
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-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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394799
Cunningham
Trypanothione reductase from L ...
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1
3
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2
1
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1
1
1
-
2
1
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-
1
1
1
2
-
-
-
-
6
1
3
-
-
2
-
-
-
-
1
-
1
3
-
2
1
-
-
-
1
1
1
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394800
Borges
Site-directed mutagenesis of t ...
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5
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8
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1
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6
-
-
-
3
-
-
-
1
-
1
3
-
3
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
5
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8
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1
-
-
6
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Cunningham
Mechanism of inhibition of try ...
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1
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1
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1
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1
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-
2
2
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1
-
-
2
-
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1
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7
2
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-
-
-
-
-
-
1
-
-
1
-
1
-
1
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-
-
1
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-
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394797
Kelly
Phenotype of recombinant Leish ...
Leishmania donovani, Trypanosoma cruzi
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2
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1
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4
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4
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-
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-
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4
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2
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1
4
-
-
-
-
5
-
-
-
-
-
3
-
-
-
1
-
-
4
-
4
-
-
-
-
-
-
-
-
-
-
-
-
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394802
El-Waer
The glutamyl binding site of t ...
Crithidia fasciculata, Trypanosoma congolense
Biochim. Biophys. Acta
1203
93-98
1993
2
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6
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2
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11
-
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-
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6
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-
-
4
-
-
-
2
-
-
4
-
-
-
-
-
-
6
-
-
-
2
-
-
-
-
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
394775
Krauth-Siegel
NADPH-dependent disulphide red ...
Crithidia fasciculata, Trypanosoma congolense, Trypanosoma cruzi
Biochem. Soc. Trans.
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315-317
1989
3
3
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1
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3
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1
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3
3
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394780
Taylor
-
Cloning by expression of a cDN ...
Crithidia fasciculata, Leishmania aethiopica, Leishmania donovani, Trypanosoma brucei, Trypanosoma congolense, Trypanosoma cruzi
Biochem. Soc. Trans.
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579-580
1989
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18
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18
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6
5
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394781
Sullivan
Expression of Trypanosoma cong ...
Crithidia fasciculata, Trypanosoma congolense, Trypanosoma cruzi
Biochemistry
28
4986-4992
1989
3
3
1
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8
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5
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5
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1
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4
3
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11
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3
1
11
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8
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1
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4
3
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2
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394782
Fairlamb
Trypanothione is the primary t ...
Crithidia fasciculata, Trypanosoma brucei
Proc. Natl. Acad. Sci. USA
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2607-2611
1989
2
2
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4
2
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2
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3
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2
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2
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4
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2
2
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2
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4
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394784
Jockers-Scheruebl
Trypanothione reductase from T ...
Trypanosoma cruzi
Eur. J. Biochem.
180
267-272
1989
1
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5
3
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1
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1
3
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2
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1
1
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394785
Henderson
Subversive substrates for the ...
Crithidia fasciculata, Trypanosoma cruzi
Proc. Natl. Acad. Sci. USA
85
5374-5378
1988
2
2
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1
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6
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2
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4
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2
2
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4
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1
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6
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2
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2
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394786
Shames
Trypanothione reductase of Try ...
Trypanosoma congolense
Biochemistry
27
5014-5019
1988
1
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1
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1
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6
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1
1
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394787
Henderson
Substrate specificity of the f ...
Crithidia fasciculata
Biochemistry
26
3023-3027
1987
1
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3
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3
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1
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1
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394788
Krauth-Siegel
Trypanothione reductase from T ...
Trypanosoma cruzi
Eur. J. Biochem.
164
123-128
1987
1
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5
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1
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1
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1
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394789
Shames
Purification and characterizat ...
Crithidia fasciculata
Biochemistry
25
3519-3526
1986
1
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1
2
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1
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