BRENDA - Enzyme Database
show all sequences of 1.8.1.10

Staphylococcus aureus coenzyme A disulfide reductase, a new subfamily of pyridine nucleotide-disulfide oxidoreductase

delCardayre, S.B.; Davies, J.E.; J. Biol. Chem. 273, 5752-5757 (1998)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
potential point of intervention in the treatment of staphylococcal infections
Staphylococcus aureus
pharmacology
anti-staphylococcal agent, possible target for the design of selective inhibitors that would interrupt the thiol metabolism of the human pathogen Staphylococcus aureus
Staphylococcus aureus
Cloned(Commentary)
Cloned (Commentary)
Organism
cdr gene encoding coenzyme A glutathione disulfide reductase is cloned, sequenced and overexpressed in Escherichia coli BL21 using the pET expression system, gene encodes a 438-amino acid polypeptide
Staphylococcus aureus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
NADPH
recombinant enzyme expressed in E. coli
Staphylococcus aureus
0.014
-
CoA disulfide
recombinant enzyme expressed in E. coli
Staphylococcus aureus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49200
-
2 * 49200, calculated from the nucleotide sequence
Staphylococcus aureus
90000
-
native molecular weight
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
CoA disulfide + NADPH
Staphylococcus aureus
central role in thiol metabolism
CoA + NADP+
-
Staphylococcus aureus
?
CoA disulfide + NADPH
Staphylococcus aureus NCTC 8325
central role in thiol metabolism
CoA + NADP+
-
Staphylococcus aureus NCTC 8325
?
Organism
Organism
UniProt
Commentary
Textmining
Staphylococcus aureus
-
strain 8325-4
-
Staphylococcus aureus NCTC 8325
-
strain 8325-4
-
Purification (Commentary)
Purification (Commentary)
Organism
purification of recombinant CoADR, expressed in Escherichia coli
Staphylococcus aureus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Staphylococcus aureus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
CoA disulfide + NADPH
substrate CoASSCoA, enzyme forms during catalysis a stable mixed disulfide intermediate with CoA
395111
Staphylococcus aureus
CoA + NADP+
-
395111
Staphylococcus aureus
?
CoA disulfide + NADPH
central role in thiol metabolism
395111
Staphylococcus aureus
CoA + NADP+
-
395111
Staphylococcus aureus
?
CoA disulfide + NADPH
substrate CoASSCoA, enzyme forms during catalysis a stable mixed disulfide intermediate with CoA
395111
Staphylococcus aureus NCTC 8325
CoA + NADP+
-
395111
Staphylococcus aureus NCTC 8325
?
CoA disulfide + NADPH
central role in thiol metabolism
395111
Staphylococcus aureus NCTC 8325
CoA + NADP+
-
395111
Staphylococcus aureus NCTC 8325
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 49200, calculated from the nucleotide sequence
Staphylococcus aureus
Synonyms
Synonyms
Commentary
Organism
CoA disulfide reductase
-
Staphylococcus aureus
CoADR
-
Staphylococcus aureus
More
subfamily of pyridine nucleotide-disulfide oxidoreductase
Staphylococcus aureus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1100
-
CoA disulfide
-
Staphylococcus aureus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein, gene with two FAD-binding regions
Staphylococcus aureus
NADPH
NADPH-dependent
Staphylococcus aureus
NADPH
gene with one NADPH-binding region
Staphylococcus aureus
Application (protein specific)
Application
Commentary
Organism
medicine
potential point of intervention in the treatment of staphylococcal infections
Staphylococcus aureus
pharmacology
anti-staphylococcal agent, possible target for the design of selective inhibitors that would interrupt the thiol metabolism of the human pathogen Staphylococcus aureus
Staphylococcus aureus
Cloned(Commentary) (protein specific)
Commentary
Organism
cdr gene encoding coenzyme A glutathione disulfide reductase is cloned, sequenced and overexpressed in Escherichia coli BL21 using the pET expression system, gene encodes a 438-amino acid polypeptide
Staphylococcus aureus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein, gene with two FAD-binding regions
Staphylococcus aureus
NADPH
NADPH-dependent
Staphylococcus aureus
NADPH
gene with one NADPH-binding region
Staphylococcus aureus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
NADPH
recombinant enzyme expressed in E. coli
Staphylococcus aureus
0.014
-
CoA disulfide
recombinant enzyme expressed in E. coli
Staphylococcus aureus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49200
-
2 * 49200, calculated from the nucleotide sequence
Staphylococcus aureus
90000
-
native molecular weight
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
CoA disulfide + NADPH
Staphylococcus aureus
central role in thiol metabolism
CoA + NADP+
-
Staphylococcus aureus
?
CoA disulfide + NADPH
Staphylococcus aureus NCTC 8325
central role in thiol metabolism
CoA + NADP+
-
Staphylococcus aureus NCTC 8325
?
Purification (Commentary) (protein specific)
Commentary
Organism
purification of recombinant CoADR, expressed in Escherichia coli
Staphylococcus aureus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
CoA disulfide + NADPH
substrate CoASSCoA, enzyme forms during catalysis a stable mixed disulfide intermediate with CoA
395111
Staphylococcus aureus
CoA + NADP+
-
395111
Staphylococcus aureus
?
CoA disulfide + NADPH
central role in thiol metabolism
395111
Staphylococcus aureus
CoA + NADP+
-
395111
Staphylococcus aureus
?
CoA disulfide + NADPH
substrate CoASSCoA, enzyme forms during catalysis a stable mixed disulfide intermediate with CoA
395111
Staphylococcus aureus NCTC 8325
CoA + NADP+
-
395111
Staphylococcus aureus NCTC 8325
?
CoA disulfide + NADPH
central role in thiol metabolism
395111
Staphylococcus aureus NCTC 8325
CoA + NADP+
-
395111
Staphylococcus aureus NCTC 8325
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 49200, calculated from the nucleotide sequence
Staphylococcus aureus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1100
-
CoA disulfide
-
Staphylococcus aureus
Other publictions for EC 1.8.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
395111
delCardayre
Staphylococcus aureus coenzyme ...
Staphylococcus aureus, Staphylococcus aureus NCTC 8325
J. Biol. Chem.
273
5752-5757
1998
-
2
1
-
-
-
-
2
-
-
2
2
-
12
-
-
1
-
-
-
1
-
4
1
3
-
-
-
1
-
-
-
3
-
-
-
-
2
1
3
-
-
-
-
-
-
2
-
-
2
2
-
-
-
1
-
-
1
-
4
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
395105
Acuna
-
Distribution of GSSG- and CoAS ...
Rattus norvegicus
Temas Bioquim. Actual. (Pina, E. , Pena, A. , Chagoya de Sanchez, V. , eds. )
93
355-359
1978
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
10
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395106
Loewen
Identification of a coenzyme A ...
Escherichia coli
Can. J. Biochem.
55
1019-1027
1977
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395107
Eriksson
The nature of the enzymatic re ...
Rattus norvegicus
FEBS Lett.
39
296-300
1974
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395108
Ondarza
CoAS-Sglutathione and GSSG red ...
Rattus norvegicus
Biochim. Biophys. Acta
341
162-171
1974
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
2
-
-
1
1
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
1
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
395012
Dyar
Rat liver levels of coenzyme A ...
Rattus norvegicus
Arch. Biochem. Biophys.
153
619-626
1972
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
2
1
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
2
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
395110
Ondarza
Characterization of a NADPH-de ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
191
239-248
1969
-
-
-
-
-
-
1
1
-
-
1
-
-
3
-
-
1
-
-
-
1
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
1
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-