BRENDA - Enzyme Database
show all sequences of 1.8.1.10

Identification of a coenzyme A--glutathione disulfide (DSI), a modified coenzyme A disulfide (DSII), and a NADPH-dependent coenzyme A--glutathione disulfide reductase in E. coli

Loewen, P.C.; Can. J. Biochem. 55, 1019-1027 (1977)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
CoA-glutathione + NADPH
Escherichia coli
metabolism of CoA-glutathione disulfide, role may be to replenish the CoA pool from CoAS-SG formed either chemically or enzymatically during cell growth, accumulation of CoAS-SG in vivo in spite of high enzyme levels indicates involvement of a control mechanism
glutathione + CoA + NADP+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activity of enzyme in crude extract increases during growth from early log phase into stationary phase and during a shift from aerobic to anaerobic growth
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
CoA-glutathione + NADPH
substrate coenzyme A-glutathione mixed disulfide, CoAS-SG, is capable of binding one molecule of Fe3+
395106
Escherichia coli
glutathione + CoA + NADP+
-
395106
Escherichia coli
ir
CoA-glutathione + NADPH
metabolism of CoA-glutathione disulfide, role may be to replenish the CoA pool from CoAS-SG formed either chemically or enzymatically during cell growth, accumulation of CoAS-SG in vivo in spite of high enzyme levels indicates involvement of a control mechanism
395106
Escherichia coli
glutathione + CoA + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
NADPH-dependent
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
NADPH-dependent
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
CoA-glutathione + NADPH
Escherichia coli
metabolism of CoA-glutathione disulfide, role may be to replenish the CoA pool from CoAS-SG formed either chemically or enzymatically during cell growth, accumulation of CoAS-SG in vivo in spite of high enzyme levels indicates involvement of a control mechanism
glutathione + CoA + NADP+
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activity of enzyme in crude extract increases during growth from early log phase into stationary phase and during a shift from aerobic to anaerobic growth
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
CoA-glutathione + NADPH
substrate coenzyme A-glutathione mixed disulfide, CoAS-SG, is capable of binding one molecule of Fe3+
395106
Escherichia coli
glutathione + CoA + NADP+
-
395106
Escherichia coli
ir
CoA-glutathione + NADPH
metabolism of CoA-glutathione disulfide, role may be to replenish the CoA pool from CoAS-SG formed either chemically or enzymatically during cell growth, accumulation of CoAS-SG in vivo in spite of high enzyme levels indicates involvement of a control mechanism
395106
Escherichia coli
glutathione + CoA + NADP+
-
-
-
?
Other publictions for EC 1.8.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
395111
delCardayre
Staphylococcus aureus coenzyme ...
Staphylococcus aureus, Staphylococcus aureus NCTC 8325
J. Biol. Chem.
273
5752-5757
1998
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2
1
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-
2
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2
2
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12
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1
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4
1
3
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2
1
2
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2
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1
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1
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4
1
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1
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395105
Acuna
-
Distribution of GSSG- and CoAS ...
Rattus norvegicus
Temas Bioquim. Actual. (Pina, E. , Pena, A. , Chagoya de Sanchez, V. , eds. )
93
355-359
1978
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1
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10
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10
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1
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-
395106
Loewen
Identification of a coenzyme A ...
Escherichia coli
Can. J. Biochem.
55
1019-1027
1977
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1
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1
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2
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395107
Eriksson
The nature of the enzymatic re ...
Rattus norvegicus
FEBS Lett.
39
296-300
1974
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1
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1
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1
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1
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1
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2
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395108
Ondarza
CoAS-Sglutathione and GSSG red ...
Rattus norvegicus
Biochim. Biophys. Acta
341
162-171
1974
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1
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1
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1
1
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1
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1
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1
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1
1
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2
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1
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395012
Dyar
Rat liver levels of coenzyme A ...
Rattus norvegicus
Arch. Biochem. Biophys.
153
619-626
1972
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2
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1
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395110
Ondarza
Characterization of a NADPH-de ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
191
239-248
1969
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