General Stability | Organism |
---|---|
unstable in crude extracts and in solutions below pH 7 | [Chlorella] fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 7% inhibition at 5 mM | [Chlorella] fusca | |
CO | - |
[Chlorella] fusca | |
cyanide | 90% inhibition at 1 mM | [Chlorella] fusca | |
EDTA | - |
[Chlorella] fusca | |
o-phenanthroline | 15% inhibition at 5 mM | [Chlorella] fusca | |
p-chloromercuribenzoate | complete inhibition at 0.1 mM | [Chlorella] fusca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 2 atoms of iron probably bound in the chromophoric group | [Chlorella] fusca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
gel filtration, SDS-PAGE | [Chlorella] fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Chlorella] fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Chlorella] fusca |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51.7 | - |
- |
[Chlorella] fusca |
Storage Stability | Organism |
---|---|
-24°C, 30% loss of activity within 4 months | [Chlorella] fusca |
4°C, 30% loss of activity within 3 weeks | [Chlorella] fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced ferredoxin | other electron donor: methyl viologen | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000 SDS-PAGE + mercaptoethanol | [Chlorella] fusca |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
enzyme in crude extract is rapidly inactivated below pH 7 | [Chlorella] fusca |