General Stability | Organism |
---|---|
repetitive freezing and thawing or prolonged dialysis produces a loss of enzyme activity | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | 9507 | - |
chloroplast | - |
Spinacia oleracea | 9507 | - |
chloroplast | - |
[Chlorella] fusca | 9507 | - |
chloroplast | - |
Cucurbita pepo | 9507 | - |
chloroplast | - |
Anabaena cylindrica | 9507 | - |
chloroplast | - |
Dunaliella tertiolecta | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
[Chlorella] fusca | |
Iron | - |
Cucurbita pepo | |
Iron | - |
Dunaliella tertiolecta | |
Iron | enzyme contains 1 tetranuclear center (4Fe-4S) and 1 siroheme, minimum of 5 Fe atoms per active enzyme molecule | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | Spinacia oleracea | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | [Chlorella] fusca | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | Cucurbita pepo | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | Anabaena cylindrica | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | Dunaliella tertiolecta | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena cylindrica | - |
- |
- |
Cucurbita pepo | - |
- |
- |
Dunaliella tertiolecta | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
[Chlorella] fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Synechococcus elongatus PCC 7942 = FACHB-805 | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Spinacia oleracea | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | [Chlorella] fusca | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Cucurbita pepo | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Anabaena cylindrica | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Dunaliella tertiolecta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
108 | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, for at least 6 months | Spinacia oleracea |
4°C, 0.1 M potassium phosphate buffer, pH 7.7, 1 week | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced ferredoxin | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
[Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
Anabaena cylindrica | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
Dunaliella tertiolecta | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Synechococcus elongatus PCC 7942 = FACHB-805 | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Anabaena cylindrica | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Dunaliella tertiolecta | ammonia + oxidized ferredoxin | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable | Spinacia oleracea |
60 | - |
10 min, more than 95% loss of activity | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Spinacia oleracea |
7 | 8 | - |
[Chlorella] fusca |
7 | 8 | - |
Cucurbita pepo |
7 | 8 | - |
Anabaena cylindrica |
7 | 8 | - |
Dunaliella tertiolecta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
siroheme | prosthetic group | Spinacia oleracea | |
siroheme | 1 mol siroheme per mol of enzyme | Spinacia oleracea |