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Literature summary for 1.7.7.1 extracted from

  • Arizmendi, J.M.; Serra, J.L.
    Purification and some properties of the nitrite reductase from the cyanobacterium Phormidium laminosum (1990), Biochim. Biophys. Acta, 1040, 237-244.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
bathophenanthroline slight Phormidium laminosum
cyanide 92% inhibition at 0.1 mM Phormidium laminosum
hydroxylamine 34% inhibition at 10 mM Phormidium laminosum
N-ethylmaleimide 47% inhibition at 1 mM Phormidium laminosum
NaN3 slight Phormidium laminosum
p-hydroxymercuribenzoate
-
Phormidium laminosum
SO32- 24% inhibition at 10 mM Phormidium laminosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
oxidized ferredoxin
-
Phormidium laminosum
0.04
-
nitrite
-
Phormidium laminosum
0.215
-
methyl viologen
-
Phormidium laminosum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Phormidium laminosum 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
gel filtration Phormidium laminosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrite + reduced ferredoxin Phormidium laminosum
-
ammonia + oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Phormidium laminosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Phormidium laminosum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
625
-
-
Phormidium laminosum

Storage Stability

Storage Stability Organism
-20°C, several months Phormidium laminosum
4°C, 1 week Phormidium laminosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NH3 + H2O + oxidized ferredoxin
-
Phormidium laminosum nitrite + reduced ferredoxin + H+
-
r
nitrite + reduced ferredoxin
-
Phormidium laminosum ammonia + oxidized ferredoxin
-
?
nitrite + reduced ferredoxin NADH Phormidium laminosum ammonia + oxidized ferredoxin
-
?
nitrite + reduced ferredoxin NADPH Phormidium laminosum ammonia + oxidized ferredoxin
-
?
nitrite + reduced ferredoxin other electron donor: methyl viologen Phormidium laminosum ammonia + oxidized ferredoxin
-
?
nitrite + reduced ferredoxin other electron donors: flavodoxin Phormidium laminosum ammonia + oxidized ferredoxin
-
?
nitrite + reduced ferredoxin not: flavin Phormidium laminosum ammonia + oxidized ferredoxin
-
?
nitrite + reduced ferredoxin other electron donor: benzyl viologen Phormidium laminosum ammonia + oxidized ferredoxin
-
?
nitrite + reduced methyl viologen
-
Phormidium laminosum NH3 + H2O + oxidized methyl viologen
-
?

Subunits

Subunits Comment Organism
monomer 1 * 54000 SDS-PAGE Phormidium laminosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
increase of activity up to 50°C Phormidium laminosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
thermostable up to 40°C Phormidium laminosum
60
-
10 min, more than 95% loss of activity Phormidium laminosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
phosphate buffer Phormidium laminosum
7.6
-
Tris-HCl buffer Phormidium laminosum

Cofactor

Cofactor Comment Organism Structure
siroheme prosthetic group Phormidium laminosum