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Literature summary for 1.7.5.1 extracted from

  • Iobbi-Nivol, C.; Santini, C.L.; Blasco, F.; Giordano, G.
    Purification and further characterization of the second nitrate reductase of Escherichia coli K12 (1990), Eur. J. Biochem., 188, 679-687.
    View publication on PubMed
Search for more literature for 1.7.5.1

General Stability

General Stability Organism
freezing in liquid nitrogen may be repeated up to six times, with a reduction of 20% of the activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
azide 5 mM, 95-98% inhibition Escherichia coli
cyanide 1 mM, 95-98% inhibition Escherichia coli
additional information p-chloromercuribenzoate (0.5 mM) or 2-heptyl-4-hydroxyquinolin N-oxide (1 mM) are almost without effect on the purified enzyme tested with reduced viologen as electron donor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 nitrate
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe the 230000 Da complex contains 13 atoms iron and 12 atoms labile sulfur/molecules Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 1 * 150000 (alphaz) + 1 * 60000 (betaz) + a b-type cytochrome subunit, SDS-PAGE Escherichia coli
150000
-
 1 * 150000 (alphaz) + 1 * 60000 (betaz) + a b-type cytochrome subunit, SDS-PAGE Escherichia coli
230000
-
 alpha(Z)beta(Z) complex, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli nitrate reductase Z expression is regulated in a manner opposite to that of nitrate reductase A. The narGHJZ operon is aerobically repressed, strongly induced by nitrate and positively regulated by the fnr gene product. The expression of narZ is anaerobically repressed, induced weakly, if at all, by nitrate and negatively regulated by the fnr gene product. The opposing regulation of these two enzymes suggests that a function of nitrate reductase Z may be to catalyse the immediate flow of electrons to nitrate during an aerobic/anaerobic transition when the bacterium is grown in the presence of nitrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is remarkably resistant to air inactivation since only 2-5% of the activity is lost after a 1 h treatment Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, slow freezing leads to a 30% loss of activity Escherichia coli
4°C, the purified preparation can be stored up to three days without inactivation Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information nitrate reductase Z expression is regulated in a manner opposite to that of nitrate reductase A. The narGHJZ operon is aerobically repressed, strongly induced by nitrate and positively regulated by the fnr gene product. The expression of narZ is anaerobically repressed, induced weakly, if at all, by nitrate and negatively regulated by the fnr gene product. The opposing regulation of these two enzymes suggests that a function of nitrate reductase Z may be to catalyse the immediate flow of electrons to nitrate during an aerobic/anaerobic transition when the bacterium is grown in the presence of nitrate Escherichia coli ?
-
 ?
additional information bromate and chlorate are substrates of the enzyme Escherichia coli ?
-
 ?
nitrate + reduced benzyl viologen
-
 Escherichia coli nitrite + oxidized benzyl viologen + H2O
-
 ?
nitrate + reduced methyl viologen
-
 Escherichia coli nitrite + oxidized methyl viologen + H2O
-
 ?

Subunits

Subunits Comment Organism
? 1 * 150000 (alphaz) + 1 * 60000 (betaz) + a b-type cytochrome subunit, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
nitrate reductase Z
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
bis(molybdopterin guanine dinucleotide)molybdenum cofactor molybdoenzyme Escherichia coli
cytochrome although the spectral studies of nitrate reductase Z reveals the presence of a b-type cytochrome subunit (1.5 mol/molecule of 230000 Da), none can be detected in the SDS-PAGE Escherichia coli