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Literature summary for 1.7.5.1 extracted from

  • Iobbi, C.; Santini, C.L.; Bonnefoy, V.; Giordano, G.
    Biochemical and immunological evidence for a second nitrate reductase in Escherichia coli K12 (1987), Eur. J. Biochem., 168, 451-459.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
KCN 1 mM, complete inhibition Escherichia coli K-12
NaN3 10 mM, complete inhibition Escherichia coli K-12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.9
-
nitrate cosubstrate: benzyl viologen Escherichia coli K-12

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Escherichia coli K-12 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mo molybdoenzyme Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli K-12 nitrate reductase Z is synthesized in small amounts, the expression of its structural genes does not seem to be induced by nitrate, repressed by oxygen or activated by the product of the fnr gene. The nitrate reductase Z in mutant LCB79/pLCB14 couples formate oxidation with nitrate reduction probably via quinones and type-b cytochromes ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information nitrate reductase Z is synthesized in small amounts, the expression of its structural genes does not seem to be induced by nitrate, repressed by oxygen or activated by the product of the fnr gene. The nitrate reductase Z in mutant LCB79/pLCB14 couples formate oxidation with nitrate reduction probably via quinones and type-b cytochromes Escherichia coli K-12 ?
-
?
additional information nitrate reductase Z is able to use both nitrate and chlorate as substrate Escherichia coli K-12 ?
-
?
nitrate + reduced benzyl viologen
-
Escherichia coli K-12 nitrite + benzyl viologen
-
?

Synonyms

Synonyms Comment Organism
nitrate reductase Z
-
Escherichia coli K-12

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
20 min, stable Escherichia coli K-12
65
-
4 min, 50% loss of activity Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Escherichia coli K-12